The use of DNA probes identifying restriction-fragment-length polymorphisms to examine the Mycobacterium avium complex
J. J. McFadden
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
For correspondence.
Search for more papers by this authorP. D. Butcher
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorJ. Thompson
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorR. Chiodini
Department of Pathology, University of Connecticut, Storrs, Connecticut 06268. USA.
Search for more papers by this authorJ. Hermon-Taylor
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorJ. J. McFadden
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
For correspondence.
Search for more papers by this authorP. D. Butcher
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorJ. Thompson
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorR. Chiodini
Department of Pathology, University of Connecticut, Storrs, Connecticut 06268. USA.
Search for more papers by this authorJ. Hermon-Taylor
Department of Surgery, St George's Hospital Medical School, London SW17 0RE. UK.
Search for more papers by this authorSummary
DNA probes were used to identify restriction-fragment-length polymorphisms (RFLPs) in DNA samples, demonstrating that the Mycobacterium avium complex could be clearly divided Into M. avium and Mycobacterium intracellulare strains. Less than 2% DNA base substitution was found between M. avium strains, whereas the M. intracellulare strains had greater than 15% base substitution. The Johne's disease bacillus, Mycobacterium paratubercuiosis (American type strain), was found to be distinguishable from the M. avium complex serotypes examined. Strain 18 was found to be identical to M. avium. The rat leprosy bacillus, Mycobacterium lepraemurium, was found to be very closely related, but not identical, to M. avium.
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