Free Access

Stimulation of capacitation and the acrosome reaction in ejaculated buffalo (Bubalus bubalis) sperm and the effects of a sperm motility factor

Corresponding Author

K. S. Sidhu

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Department of Zoology, Punjab Agriculatural University, Ludhiana-141004, India.Search for more papers by this author

R. Sundhey,

R. Sundhey

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Search for more papers by this author

S. S. Guraya,

S. S. Guraya

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Search for more papers by this author

K. S. Sidhu,

Corresponding Author

K. S. Sidhu

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Department of Zoology, Punjab Agriculatural University, Ludhiana-141004, India.Search for more papers by this author

R. Sundhey,

R. Sundhey

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Search for more papers by this author

S. S. Guraya,

S. S. Guraya

ICMR Advance Centre in Reproductive Biology, Department of Zoology, College of Basic Sciences & Humanities, Punjab Agricultural University, Ludhiana-141004, India

Search for more papers by this author

First published: August 1984

https://doi.org/10.1111/j.1365-2605.1984.tb00789.x

Citations: 15

About

PDF

Tools

Share a link

Email
Wechat
Bluesky

Abstract

The conditions for stimulation of in vitro capacitation and the acrosome reaction of ejaculated buffalo sperm has been determined. Washed ejaculated sperm were successfully capacitated in BWW medium supplemented with bovine serum albumin (BSA) and a sperm motility factor(s) (SMF(s) isolated from the adrenal glands of rats. The acrosome reaction was induced in capacitated sperm by introducing Ca⁺⁺ ions (final concentration 5 mM) into the medium. Supplementation of BWW medium with SMF(s) in the presence of BSA significantly increased the percentage of sperm showing capacitation and the acrosome reaction. SMF(s) also significantly increased the percentage motility, the percentage forward motility and maintained a higher percentage of live sperm in BWW medium under the conditions used in this study. The significance of the present findings is discussed.

References

Ain-Shoka A A & Buckner C K (1978): Studies on the inhibition of beta-adrenergic receptor agonists of cAMP phosphodiesterase activity of rat heart). Arch Int Pharm 239: 269.
Google Scholar
Barros C E (1968b): In vitro capacitation of golden hamster spermatozoa with fallopian tube fluid of the mouse and rat). J Reprod Fertil 17: 203.
10.1530/jrf.0.0170203
CAS PubMed Web of Science® Google Scholar
Barros C E. Arrau J E & Herrera JE (1972): Induction of the acrosome reaction of golden hamster spermatozoa with blood serum collected at different stages of the oestrous cycle). J Reprod Fertil 28: 67.
10.1530/jrf.0.0280067
CAS PubMed Web of Science® Google Scholar
Barros C E & Garavagno A. (1970): Capacitation of hamster spermatozoa with blood sera). J Reprod Fertil 22: 381.
10.1530/jrf.0.0220381
Web of Science® Google Scholar
Bavister B D, Yanagimachi R B & Robert J., Teichman J. (1976): Capacitation of hamster spermatozoa with adrenal gland extract). Biol Reprod 14: 219.
10.1095/biolreprod14.2.219
CAS PubMed Web of Science® Google Scholar
Bedford J M (1970): Sperm capacitation and fertilization in mammals). Biol Reprod Suppl 2: 128.
10.1095/biolreprod2.Supplement_2.128
CAS PubMed Google Scholar
Biggers J D. Written W R & Whittingham D G (1971): The culture of mouse embryos in vitro. In: J C Daniel (ed). In Methods of Mammalian Embryology, p 101.
Google Scholar
Byrd W. (1981): In vitro capacitation and the chemically induced acrosome reaction in bovine spermatozoa. J Exp Zool 215 (1): 35.
10.1002/jez.1402150105
CAS PubMed Web of Science® Google Scholar
Goren E M & Rosen O M (1972): Inhibition of a cyclic nucleotides phosphodiesterase from beef heart by catecholamines and related compounds). Molec Pharm 8: 380.
CAS PubMed Web of Science® Google Scholar
Gornett L E & Meizel S. (1978): Stimulation of an in vitro activation and acrosome reaction of hamster sperm by catecholamines). Proc Nat Acad-Sci USA 75: 4945.
Google Scholar
Fagan J B & Racker E. (1977): Reversible inhibition of (Na⁺, K⁺) ATPase by Mg⁺⁺, adenosine triphosphate and K⁺). Biochem 16: 152.
10.1021/bi00620a026
CAS PubMed Web of Science® Google Scholar
Guraya S S & Sidhu K S (1975): Histochemical observations of buffalo spermatozoa). Ind J Anim Sci 45: 923.
Google Scholar
Gwatkin R B L (1977): Fertilization Mechanism in Man and Mammals. 161 p. N Y Plenum Press.
10.1007/978-1-4615-8804-7
Google Scholar
Gwatkin R B L & Anderson F F (1969): Capacitation of hamster spermatozoa by bovine follicular fluid). Nature (London) 224: 1.
10.1038/2241111a0
CAS Web of Science® Google Scholar
Hexus T. (1977): The effect of catecholamines on transport (Na⁺, K⁺) adenosine triphosphatase). Biochem Pharm 26: 1221.
10.1016/0006-2952(77)90109-5
CAS PubMed Web of Science® Google Scholar
Kirton K T & Hafs H D (1965): Sperm capacitation by uterine fluid or by β-amylase in vitro). Science 150: 618.
10.1126/science.150.3696.618
CAS PubMed Web of Science® Google Scholar
Lui C W, Cornet L E & Meizel S. (1977): Identification of the bovine follicular fluid protein involved in the in vitro induction of the hamster sperm acrosome reaction). Biol Reprod 17: 34.
10.1095/biolreprod17.1.34
CAS PubMed Web of Science® Google Scholar
Marsny R J & Meizel S. (1979): Inhibition of hamster sperm acrosome reaction by inhibitors of Na⁺-K⁺ATPase. Abstracts of 1979. American Society of Cell Biology Meetings. J Cell Biol 83. 206a Abstr F D 1029.
Google Scholar
Marsny R J & Meizel S. (1980): Evidence suggesting a role for cyclic nucleotides in acrosome reaction by hamster sperm in vitro). J Exp Zool 211: 153.
10.1002/jez.1402110205
CAS PubMed Web of Science® Google Scholar
Meizel S. & Working P K (1980): Further evidence suggesting the hormonal stimulation of hamster sperm acrosome reactions by catecholamines in vitro). Biol Reprod 22: 211.
10.1095/biolreprod22.2.211
CAS PubMed Web of Science® Google Scholar
Oliphant G. (1976): Removal of sperm bound seminal plasma components as a pre-requisite to induction of the rabbit acrosome reaction). Fertil Steril 27: 28.
CAS PubMed Web of Science® Google Scholar
Sidhu K S & Guraya S S (1979): Morphological, biochemical and physiological studies on the preservation of buffalo spermatozoa in different modified dilutors: effects of chloroquine-diphosphate. Ann Biol Anim Biochem Biophys 19(1A): 45.
10.1051/rnd:19790103
CAS Web of Science® Google Scholar
Snedecor G W (1956): Statistical Methods Applied to Experiments in Agriculture and Biology. 534 p.
Google Scholar
Yanagimachi R. (1969a): In vitro acrosome reaction and capacitation of golden hamster spermatozoa by bovine follicular fluid, and its functions). J Exp Zool 170: 269.
10.1002/jez.1401700304
Web of Science® Google Scholar
Yanagimachi R. (1969b): In vitro capacitation of hamster spermatozoa by follicular fluid). J Reprod Fertil 18: 275.
10.1530/jrf.0.0180275
PubMed Web of Science® Google Scholar
Yanagimachi R. (1970a): The movement of golden hamster spermatozoa before and after capacitation). J Reprod Fertil 23: 193.
10.1530/jrf.0.0230193
PubMed Web of Science® Google Scholar
Yanagimachi R. (1970b): In vitro capacitation of hamster spermatozoa by homologous and heterologous blood sera). Biol Reprod 3: 147.
10.1093/biolreprod/3.2.147
CAS PubMed Google Scholar

Citing Literature

Volume7, Issue4

August 1984

Pages 324-333

Stimulation of capacitation and the acrosome reaction in ejaculated buffalo (Bubalus bubalis) sperm and the effects of a sperm motility factor

Abstract

References

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

Stimulation of capacitation and the acrosome reaction in ejaculated buffalo (Bubalus bubalis) sperm and the effects of a sperm motility factor

Abstract

References

Citing Literature

References

Related

Information