Differential upregulation of intercellular adhesion molecule-1 in coeliac disease
R. P. STURGESS
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorJ. C. MACARTNEY
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorM. W. MAKGOBA
Division of Molecular Endocrinology, RPMS, Hammersmith Hospital, London, England
Search for more papers by this authorC.-H. HUNG
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorD. O. HASKARD
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorCorresponding Author
P. J. CICLITIRA
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Dr P. J. Ciclitira, Division of Pharmacology, The Rayne Institute, UMDS, St Thomas's Hospital, London SE1 7EH, UK.Search for more papers by this authorR. P. STURGESS
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorJ. C. MACARTNEY
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorM. W. MAKGOBA
Division of Molecular Endocrinology, RPMS, Hammersmith Hospital, London, England
Search for more papers by this authorC.-H. HUNG
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorD. O. HASKARD
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Search for more papers by this authorCorresponding Author
P. J. CICLITIRA
Divisions of Pharmacology and Medicine, The Rayne Institute, UMDS and Department of Pathology, St Thomas's Hospital, London, England
Dr P. J. Ciclitira, Division of Pharmacology, The Rayne Institute, UMDS, St Thomas's Hospital, London SE1 7EH, UK.Search for more papers by this authorSUMMARY
The expression of intercellular adhesion molecule-l (ICAM-1) was studied on peroral jejunal biopsies from patients with coeliac disease. The biopsies from untreated patients exhibited greater staining of the superficial lamina propria cells compared with treated patients and controls. A gluten challenge in treated patients produced an altered staining pattern within 2 h. The results demonstrate the role of ICAM-1 expression in coeliac disease, providing further evidence for the role of lamina propria cells in the pathogenesis of this condition.
REFERENCES
- Adams, D.H., Hubscher, S.G., Shaw, J., Rothlein, R. & Neuberger, J.M. (1989) Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet, ii, 1122.
- Anand, B.S., Piris, T., Jerrome, D.W., Offord, R.E. & Truelove, S.C. (1981) The timing of histological damage following a single challenge with gluten in treated coeliae disease. Q. J. Med 50, 83.
- Bailey, D.S., Freedman, A.R., Price, S.C., Chescoe, D. & Ciclitira, P.J. (1989) Early biochemical responses of the small intestine of coeliac patients to wheat gluten. Hut. 30, 78.
- Brandtzaeg, P., Halstensem, T.S., Kktt, K., Kracci, P., Kvale, D., Rognum, T.O., Scott, H. & Sollid, L.M. (1989) lmmunobiology and immunopathology of human gut mucosa: humoral immunity and intraepithelial lymphocytes. Gastroenterolagy, 91, 1562.
- Ciclitira, P.J., Ellis, H.J., Wood, G.M., Howdle, P.D. & Losowsky, M.S. (1986) Secretion of gliadin antibody by coeliac jejunal mucosal biopsies cultured in vitro. Clin. exp. Immunol. 64, 119.
- Dustin, M.L., Staunton, D.E. & Springer, T.A. (1988) Supergene families meet in the immune system. Immunol. Today. 9, 213.
- Dustin, M.L., Rothlkin, R., Bhan, A.K., Dinarello, C.A. & Springer, T.A. (1986) Induction by IL1 and interferon, tissue distribution, biochemistry and function of a natural adherence molecule (ICAM-I). J. Immunol. 137, 245.
- Ferguson, A., Macdonald, T.T., McClure, J.P. & Holden, R.J. (1975) Cell-mediated immunity to gliadin within the small intestinal mucosa in coeliac disease. Lancet, i, 895.
- Ferguson, A. & Murray, D. (1971) Quantitation of intraepithelial lymphocytes in human jejunum. Gut, 12, 988.
- Freedman, A.R., Galfre, G., Gal, E., Ellis, H.J. & Ciclitira, P.J. (1988) Western immunoblotting of cereal proteins with monoclonal antibodies to wheat gliadin to investigate coeliac disease. Int. Arch. Allergy appl. Immunol. 85, 346.
- Griffiths, C.E.M., Barrison, I.G., Leonard, J.N., Caun, K., Valdimarsson, H. & Fry, L. (1988) Preferential activation of CD4 T lymphocytes in the lamina propria of gluten-sensitive enteropathy. Clin. exp. Immunol. 72, 280.
- Hsu, S.M. & Raine, L. (1981) Protein A, avidin and biotin in immunohislochemistry. J. Histochem. Cytochem. 29, 1349.
- Jenkins, D., Goodall, A. & Scott, B.B. (1986) T-lymphocyte populations in normal and coeliac small intestinal mucosa defined by monoclonal antibodies. Gut. 27, 1330.
- Makgoba, M.W., Sanders, M.F., & Shaw, S. (1989) The CD2-LFA-3 and LFA-1 ICAM-1 pathways: relevance to T-cell recognition. Immunol Today, 10, 417.
- Makgoba, M.W., Sanders, M.E., Ginther Luce, G.E., Dustin, M.L., Springer, T.A., Clark, E.A., Mannoni, P. & Shaw, S. (1988) ICAM-1 a ligand for LFA-1 dependent adhesion of B.T, and myeloid cells. Nature. 331, 86.
- Marsh, M.N. (1980) Studies of intestinal lymphoid tissue. III. Quantitative analysis of epithelial lymphocytes in the small intestine of human control subjects and patients with coeliac sprue. Gastroenterology, 79, 481.
- Monk, T., Spencer, J. Cerf-Bensussan, N. & Macdonald, T.T. (1988) Stimulation of mucosal T cells in situ with the anti-CD3 antibody: location of the activated T cells and their distribution within the mucosal environment. Clin. exp. Immunol. 74, 2l6.
- Shaw, S. & Luce, G.G. (1987) The lymphocyte lunclion-associated antigen (LFA)-1 and CD2/LFA-3 pathways of antigen-independent human T cell adhesion. J. Immunol. 139, 1037.
- Singer, K.H. & Haynes, B.F. (1987) Epithelial-thymocyte interactions in human thymus. Hum. Immunol. 20, 127.
- Singer, K.H., Tuck, D.T., Sampson, H.A. & Hall, R.P. (1989) Epidermal keratinocytes express the adhesion molecule intercellular adhesion molecule-1 in inflammatory dermaloses. J. invest. Dermatol. 92, 746.
- Spencer, J., Isaacson, P.G., Diss, T.C. & Macdonald, T.T. (1989a) Expression of disulphide-linked and non-disulphide-linked forms of the T-cell receptor γδ heterodimer in human intraepithelial lymphocytes. Eur. J. Immunol. 19, 1335.
- Spencer, J., Macdonald, T.T., Diss, T.C, Walker-Smith, J.A., Ciclitira, P.J. & Isaacson, P.G. (1989b). Changes in intraepithelial lymphocyte subpopulations in coeliac disease and enteropathy associated T-cell lymphoma (malignant histiocytosis of the intestine). Gut. 30, 339.
- Springer, T.A., Dustin, M.L., Kishimoto, K. & Marlin, S.D. (1987). The lymphocyte function-associated LFA-1. CD2 and LFA-3 molecules: cell adhesion receptors of the immune system. Annu. Rev. Immunol. 5, 223.
- Weetman, A.P., Cohen, S., Makgoba, M.W. & Borysiewicz, L.K. (1989) Expression of an intercellular adhesion molecule, ICAM-1. by human thyroid cells. J. Endocrinol. 122, 185.
