Full Access
Differential Radiation Response Amongst Proliferating Epithelial Cells
C. S. Potten,
S. E. Al-Barwari,
J. Searle,
Corresponding Author
C. S. Potten
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Dr C. S. Potten, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX.Search for more papers by this authorS. E. Al-Barwari
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Search for more papers by this authorJ. Searle
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Department of Pathology, University of Queensland, Medical School, Herston, Brisbane, Australia.
Search for more papers by this authorC. S. Potten,
S. E. Al-Barwari,
J. Searle,
Corresponding Author
C. S. Potten
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Dr C. S. Potten, Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX.Search for more papers by this authorS. E. Al-Barwari
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Search for more papers by this authorJ. Searle
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester
Department of Pathology, University of Queensland, Medical School, Herston, Brisbane, Australia.
Search for more papers by this authorABSTRACT
Tissue irradiation results in both reproductive and histologically evident cell death. the correlation between these is poor. Irrespective of dose and fraction sterilized, many cells behave as if unirradiated (cell cycle and maturation activity). the fraction of histologically observable dead cells is usually less than 0.1, with most in the intestine being positioned in the presumptive stem cell region. the data strongly suggest different epithelial sub-populations with differing radioresponses. These may be the stem and differentiated proliferative cells.
REFERENCES
- Al-barwari, S.E. & Potten, C.S. (1976) Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells. Int. J. Radiat. Biol. 30, 201.
- Al-dewachi, H.S., Wright, N.A., Appleton, D.R. & Watson, A.J. (1975) Cell population kinetics in the mouse jejunal crypt. Virchows Arch. B. Cell Path. 18, 225.
- Boarder, T.A. & Blackett, N.M. (1976) the proliferative status of intestinal epithelial clonogenic cells: sensitivity to S phase specific cytotoxic agents. Cell Tissue Kinet. 9, 589.
- Cairnie, A.B., Lamerton, L.F. & Steel, G.G. (1965) Cell proliferation studies in the intestinal epithelium of the rat. 1. Determination of the Kinetic Parameters. Exp. Cell Res. 39, 528.
- Cheng, H. & Leblond, C.P. (1974) Origin, Differentiation and Renewal of Four Main Epithelial Cell Types in the mouse small intestine. V. Unitarian theory of the origin of the four epithelial cell types. Amer. J. Anat. 141, 537.
- Denekamp, J., Emery, E.W. & Field, S.B. (1971) Response of mouse epidermal cells to single and divided doses of fast neutrons. Radiat. Res. 45, 80.
- Denekamp, J., Stewart, F.A. & Douglas, B.G. (1976) Changes in the proliferation rate of mouse epidermis after irradiation: continuous labelling studies. Cell Tissue Kinet. 9, 19.
- Dethlefsen, L.A. & Riley, R.M. (1973) Hydroxyurea effects in the C3H mouse I. Duodenal crypt cell kinetics. Cell Tissue Kinet. 6, 3.
- Emery, E.W., Denekamp, J., Ball, M.M. & Field, S.B. (1970) Survival of mouse skin epithelial cells following single and divided doses of X-rays. Radiat. Res. 41, 450.
- Etoh, H., Taguchi, Y.H. & Tabachnick, J. (1975) Movement of beta-irradiated epidermal basal cells to the spinous-granular layers in the absence of cell division. J. invest. Derm. 64, 431.
-
Fowler, J.F. &
Denekamp, J. (1976) Regulation of epidermal stem cells.
Stem Cells of Renewing Cell Populations, (Ed. by
A.B. Cairnie,
P.K. Lala and
D.G. Osmond), p. 117. Academic Press,
N.Y.
.
10.1016/B978-0-12-155050-9.50017-1 Google Scholar
- Galjaard, H. & Bootsma, D. (1969) the regulation of cell proliferation and differentiation in intestinal epithelium. II. A quantitative histochemical and autoradiographic study after low doses of X-irradiation. Exp. Cell Res. 58, 79.
- Gelfant, S. (1966) Patterns of cell division: the demonstration of discrete cell populations. Methods in Cell Physiology. 2 (Ed. by D. M. Prescott), p. 359. Academic Press.
- Gelfant, S. & Candelas, C.C. (1975) Regulation of epidermal mitosis. J. invest. Derm. 59, 7.
- Hamilton, E. & Potten, C.S. (1972) Influence of hair plucking on the turnover time of the epidermal basal layer. Cell Tissue Kinet. 5, 505.
- Hashimoto, K. (1976) Apoptosis in lichen planus and several other dermatoses. Acta derm.-venereol. (Stockholm), 56, 187.
- Hegazy, M.A.H. & Fowler, J.F. (1973) Cell population kinetics and desquamation skin reactions in plucked and unplucked mouse skin. II. Irradiated skin. Cell Tissue Kinet. 6, 587.
- Hennings, H. & Devik, F. (1971) Comparison of cytotoxicity of hydroxyurea in normal and rapidly proliferating epidermis and small intestine in mice. Cancer Res. 31, 277.
- Hooper, C.E.S. (1956) Cell turnover in epithelial populations. J. Histochem. Cytochem. 4, 531.
- Howard, A. & Pelc, S.R. (1953) Synthesis of desoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity, Suppl. 6, 261.
- Kerr, J.F.R. (1971) Shrinkage necrosis: a distinct mode of cellular death. J. Pathol. 105, 13.
- Kerr, J.F.R., Wyllie, A.H. & Currie, A.R. (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Brit. J. Cancer, 26, 239.
- Krishan, A. & Frei, E. (1975) Morphological basis for the cytotoxic effect of vinblastine and vincristine on cultured human leukaemic lymphoblasts. Cancer Res. 35, 497.
- Leblond, C.P. & Cheng, H. (1976) Identification of stem cells in the small intestine of the mouse. Stem Cells of Reviewing Cell Populations, (Ed. by A. B. Cairnie, P. K. Lala and D. G. Osmond), Academic Press N.Y.
- Olson, R.L. & Everett, M.A. (1975) Epidermal apoptosis: cell deletion by phagocytosis. J. Cutaneous Pathol. 2, 53.
- Phillips, F.S., Sternberg, S.S., Schwartz, H.S., Cronin, A.P., Sodergren, J.E. & Vidal, P.M. (1967) Hydroxyurea. 1. Acute cell death in proliferating tissues in rats. Cancer Res. 27, 61.
- Potten, C.S. (1974) the epidermal proliferative unit: the possible role of the central basal cell. Cell Tissue Kinet. 7, 77.
- Potten, C.S. (1975a) Epidermal cell production rates. J. invest. Derm. 65, 488.
- Potten, C.S. (1975b) Kinetics and possible regulation of crypt cell populations under normal and stress conditions. Bull. du Cancer, 62, 419.
-
Potten, C.S. (1976) Small intestinal crypt stem cells.
Stem cells of Renewing Cell Populations. Academic Press. (Ed. by
A. B. Cairnie,
P. K. Lala and
D. G. Osmond), p. 91. Academic Press,
N.Y.
.
10.1016/B978-0-12-155050-9.50015-8 Google Scholar
- Potten, C.S. (1977a) Epithelial Proliferative Subpopulations. Stem cells and Tissue Homeostasis, (Ed. by B. I. Lord, C. S. Potten and R. J. Cole), Cambridge University Press. (In press.).
- Potten, C.S. (1977b) the cellular and tissue response of skin to single doses of ionising radiation. Curr. Topics Radiat. Res. (In press.).
- Potten, C.S. & Hendry, J.H. (1973) Clonogenic cells and stem cells in epidermis. Int. J. Rad. Biol. 24, 537.
- Potten, C.S. & Hendry, J.H. (1975) Differential regeneration of intestinal proliferative cells and cryptogenic cells after irradiation. Int. J. Rad. Biol. 27, 413.
- Potten, C.S., Kovacs, L. & Hamilton, E. (1974) Continuous labelling studies on mouse skin and intestine. Cell Tissue Kinet. 7, 271.
- Searle, J., Lawson, T.A., Abbott, P.J., Harmon, B. & Kerr, J.F.R 1975) an electron-microscope study of the mode of cell death induced by cancer-chemotherapeutic agents in populations of proliferating normal and neoplastic cells. J. Pathol. 116, 129.
- Searle, J. (1975) Correspondence. Cancer Res. 35, 2900.
- Testa, N.G., Hendry, J.H. & Lajtha, L.G. (1973) the response of mouse haemopoietic colony formers to acute or continuous gamma irradiation. Biomedicine, 19, 183.
- Tsubouchi, S. & Matsuzawa, T. (1974) Rapid Radiation cell death and cell proliferation in intestinal epithelium after 1000 rad irradiation. Radial. Res. 57, 451.
- Withers, H.R. (1967a) the dose-survival relationship for irradiation of epithelial cells of mouse skin. Brit. J. Radiol. 40, 187.
- Withers, H.R. (1967b) the effect of oxygen and anaesthesia on radio-sensitivity in vivo of epithelial cells in mouse skin. Brit. J. Radiol. 40, 335.
- Withers, H.R. & Elkind, M.M. (1969) Radiosensitivity and fractionation response of crypt cells of mouse jejunum. Radial. Res. 38, 598.
- Withers, H.R. & Elkind, M.M. (1970) Microcolony survival assay for cells of mouse intestinal mucosa exposed to radiation. Int. J. Radial. Biol. 17, 261.
- Wright, N.A. (1977) the cell population kinetics of repopulating cells in the intestine. Stem Cells and Tissue Homeostasis, (Ed. by B. I. Lord, C. S. Potten and R. J. Cole), Cambridge University Press. (In press.).
Citing Literature
