Integrated profiling of diffuse large B-cell lymphoma with 7q gain
Ekaterina Chigrinova
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorMichael Mian
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Division of Hematology, Azienda Ospedaliera S. Maurizio, Bolzano/Bozen, Italy
Search for more papers by this authorYulei Shen
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorTimothy C. Greiner
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorWing C. Chan
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorJulie M. Vose
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorGiorgio Inghirami
Department of Pathology and Center for Experimental Research and Medical Studies (CeRMS), University of Turin, Turin
Search for more papers by this authorAnnalisa Chiappella
Department of Oncology and Hematology, Hematology 2 Section, AOU San Giovanni Battista, Turin
Search for more papers by this authorLuca Baldini
Hematology/Bone Marrow Transplantation Unit, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, University of Milan, Milan
Search for more papers by this authorMaurilio Ponzoni
Unit of Lymphoid Malignancies, San Raffaele H Scientific Institute, Milan
Search for more papers by this authorAndrés J. M. Ferreri
Unit of Lymphoid Malignancies, San Raffaele H Scientific Institute, Milan
Search for more papers by this authorSilvia Franceschetti
Division of Hematology, Department of Clinical and Experimental Medicine and BRMA, Amedeo Avogadro University of Eastern Piedmont, Novara
Search for more papers by this authorGianluca Gaidano
Division of Hematology, Department of Clinical and Experimental Medicine and BRMA, Amedeo Avogadro University of Eastern Piedmont, Novara
Search for more papers by this authorAlessandra Tucci
Department of Pathology, University of Brescia, I Servizio di Anatomia Patologica, and Division of Hematology, Spedali Civili di Brescia, Brescia, Italy
Search for more papers by this authorFabio Facchetti
Department of Pathology, University of Brescia, I Servizio di Anatomia Patologica, and Division of Hematology, Spedali Civili di Brescia, Brescia, Italy
Search for more papers by this authorThierry Lazure
Departments of Internal Medicine and of Pathology, University Hospital of Bicêtre, AP/HP, Le Kremlin Bicêtre, France
Search for more papers by this authorOlivier Lambotte
Departments of Internal Medicine and of Pathology, University Hospital of Bicêtre, AP/HP, Le Kremlin Bicêtre, France
Search for more papers by this authorSantiago Montes-Moreno
Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
Search for more papers by this authorMiguel A. Piris
Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
Search for more papers by this authorEmanuele Zucca
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorIvo Kwee
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Dalle Molle Institute for Artificial Intelligence, Manno, Switzerland
Search for more papers by this authorFrancesco Bertoni
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorEkaterina Chigrinova
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorMichael Mian
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Division of Hematology, Azienda Ospedaliera S. Maurizio, Bolzano/Bozen, Italy
Search for more papers by this authorYulei Shen
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorTimothy C. Greiner
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorWing C. Chan
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorJulie M. Vose
Department of Pathology and Microbiology, University of Nebraska, Omaha, NE, USA
Search for more papers by this authorGiorgio Inghirami
Department of Pathology and Center for Experimental Research and Medical Studies (CeRMS), University of Turin, Turin
Search for more papers by this authorAnnalisa Chiappella
Department of Oncology and Hematology, Hematology 2 Section, AOU San Giovanni Battista, Turin
Search for more papers by this authorLuca Baldini
Hematology/Bone Marrow Transplantation Unit, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, University of Milan, Milan
Search for more papers by this authorMaurilio Ponzoni
Unit of Lymphoid Malignancies, San Raffaele H Scientific Institute, Milan
Search for more papers by this authorAndrés J. M. Ferreri
Unit of Lymphoid Malignancies, San Raffaele H Scientific Institute, Milan
Search for more papers by this authorSilvia Franceschetti
Division of Hematology, Department of Clinical and Experimental Medicine and BRMA, Amedeo Avogadro University of Eastern Piedmont, Novara
Search for more papers by this authorGianluca Gaidano
Division of Hematology, Department of Clinical and Experimental Medicine and BRMA, Amedeo Avogadro University of Eastern Piedmont, Novara
Search for more papers by this authorAlessandra Tucci
Department of Pathology, University of Brescia, I Servizio di Anatomia Patologica, and Division of Hematology, Spedali Civili di Brescia, Brescia, Italy
Search for more papers by this authorFabio Facchetti
Department of Pathology, University of Brescia, I Servizio di Anatomia Patologica, and Division of Hematology, Spedali Civili di Brescia, Brescia, Italy
Search for more papers by this authorThierry Lazure
Departments of Internal Medicine and of Pathology, University Hospital of Bicêtre, AP/HP, Le Kremlin Bicêtre, France
Search for more papers by this authorOlivier Lambotte
Departments of Internal Medicine and of Pathology, University Hospital of Bicêtre, AP/HP, Le Kremlin Bicêtre, France
Search for more papers by this authorSantiago Montes-Moreno
Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
Search for more papers by this authorMiguel A. Piris
Molecular Pathology Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain
Search for more papers by this authorEmanuele Zucca
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorIvo Kwee
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Dalle Molle Institute for Artificial Intelligence, Manno, Switzerland
Search for more papers by this authorFrancesco Bertoni
Laboratory of Experimental Oncology and Lymphoma Unit, Oncology Institute of Southern Switzerland (IOSI), Bellinzona, Switzerland
Search for more papers by this authorSummary
To characterize diffuse large B-cell lymphoma (DLBCL) with chromosome 7 gains, we combined clinical data with genomic, RNA and miRNA profiling. Gains were associated with age >60 years, female gender, a trend for higher complete response rate, lower death rate, and better overall survival in patients treated with R-CHOP. Lesions were inversely associated with bone marrow involvement and number of extra-nodal sites. Differentially expressed transcripts were enriched of genes belonging to specific pathways and miRNAs targets. MIR96, MIR182, MIR589, MIR25 were shown significantly up-regulated in 7q+ DLBCL by real-time PCR.
Supporting Information
Fig S1. Frequency plot of gains (up) and losses (down) observed in 166 DLBCL patients according to the presence or absence of 7q gain.
Table SI. Association between presence of 7q gain and other concomitant genomic lesions among 166 cases of DLBCL as evaluated by applying Fisher’s exact test (P-value) followed by multiple test correction (q-value).
Table SII. Clinical characteristics at time of diagnosis of 166 patients affected by DLBCL, according to presence or absence of 7q gain.
Table SIII.Transcripts differentially expressed between DLBCL cases with and without 7q gain as evaluated by ANOVA analysis followed by multiple test correction with a false discovery rate of 0.15.
Table SIV. Deregulated pathways in 7q+ DLBCL affecting the down-regulated in DLBCL cases with and without 7q gain based upon GSEA.
Table SV. Possible miRNA affecting the genes down- or up-regulated in DLBCL cases with and without 7q gains based upon GSEA.
Table SVI. Comparison of the expression levels of miRNAs mapped on chromosome 7 between DLBCL cases with and without 7q gain, and between ABC and GCB-DLBCL.
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| BJH_8628_sm_FigS1.pdf377.8 KB | Supporting info item |
| BJH_8628_sm_TablesS1-S2-S4-S6.pdf134.5 KB | Supporting info item |
| BJH_8628_sm_TableS3.xls91 KB | Supporting info item |
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