Establishment and characterization of a plasma cell leukaemia cell line dependent for growth on IL-6 and a bi-phenotypic subclone dependent upon both IL-3 and IL-6
Corresponding Author
M. Kobayashi
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Dr M. Kobayashi, The Third Department of Internal Medicine, Hokkaido University School of Medicine, Nishi-7, Kita-15. Kita-ku, Sapporo, Japan.Search for more papers by this authorT. Miyagishima
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorM. Imamura
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorS. Maeda
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorY. Gotohda
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorH. Iwasaki
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorK. Sakurada
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorT. Miyazaki
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorCorresponding Author
M. Kobayashi
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Dr M. Kobayashi, The Third Department of Internal Medicine, Hokkaido University School of Medicine, Nishi-7, Kita-15. Kita-ku, Sapporo, Japan.Search for more papers by this authorT. Miyagishima
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorM. Imamura
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorS. Maeda
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorY. Gotohda
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorH. Iwasaki
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorK. Sakurada
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorT. Miyazaki
The Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan
Search for more papers by this authorAbstract
Summary A human plasma cell leukaemia cell line (HSM-2) and a subclone (HSM-2.3) have been established from the bone marrow of a patient with bi-phenotypic leukaemia. Proliferation assays using a variety of cytokines demonstrated that HSM-2 proliferated in response to recombinant interleukin-6 (rIL-6), but did not respond to rIL-1, rIL-2, rIL-3, rIL-4, rIL-5, recombinant granulocyte-colony stimulating factor (rG-CSF), or recombinant granulocyte-macrophagecolony stimulating factor (rGM-CSF), and that HSM-2.3 responded to rIL-3 and rIL-6. HSM-2 expressed the CD38 (OKT10), PCA-1, cytoplasmic-IgM, and surface kappa light chain. HSM-2.3 expressed the CD14 (My4), CD33 (My9). CD38 (OKT10), CD19 (B4), CD24 (OKB2), CD10 (J5), PCA-1. HSM-2 and HSM-2.3 are useful tools for analysing the possible role of IL-3 and IL-6 in the oncogenesis of plasma cell leukaemia.
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