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A routine immuno-electron microscopic technique for localizing an auto-antibody on epidermal basement membrane
C. PROST,
L. DUBERTRET,
M. FOSSE,
J. WECHSLER,
R. TOURAINE,
C. PROST
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorCorresponding Author
L. DUBERTRET
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Professor L. Dubertret, Dermatology, Hôpital Henri Mondor, 94OIO Creteil, France.Search for more papers by this authorM. FOSSE
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorJ. WECHSLER
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorR. TOURAINE
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorC. PROST,
L. DUBERTRET,
M. FOSSE,
J. WECHSLER,
R. TOURAINE,
C. PROST
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorCorresponding Author
L. DUBERTRET
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Professor L. Dubertret, Dermatology, Hôpital Henri Mondor, 94OIO Creteil, France.Search for more papers by this authorM. FOSSE
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorJ. WECHSLER
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorR. TOURAINE
Laboratoire de Dermatologie, Hôpital Henri Mondor 94010 Creteil, France
Search for more papers by this authorSUMMARY
A new diagnostic technique is described, in which 0.7 mm thick slices of skin are freshly cut, throughly washed, slightly fixed and directly incubated in peroxidase-labelled antibodies. This easy technique allows routine immuno-electron microscopic diagnosis of subepidermal auto-immune bullous diseases, with excellent morphological results.
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