Induction of DNA Replication and Cell Growth in Rat Liver by Obstructive Jaundice
Masaki Terasaki
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorHiroshi Kuriki
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorYuji Nimura
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorShigehiko Shionoya
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorKiyohide Kojima
Laboratoty of Cancer Cell Biology, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorCorresponding Author
Shonen Yoshida
Laboratoty of Cancer Cell Biology, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
To whom correspondence should be addressed.Search for more papers by this authorMasaki Terasaki
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorHiroshi Kuriki
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorYuji Nimura
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorShigehiko Shionoya
The First Department of Surgery, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorKiyohide Kojima
Laboratoty of Cancer Cell Biology, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
Search for more papers by this authorCorresponding Author
Shonen Yoshida
Laboratoty of Cancer Cell Biology, Institute for Disease Mechanism and Control, Nagoya University School of Medicine, 65 Tsurumai-cho, Showa-kn, Nagoya 466
To whom correspondence should be addressed.Search for more papers by this authorAbstract
Obstructive jaundice, produced by ligating the common bile duct, induced a transient DNA replication followed by cell proliferation in rat liver. At 48 h after the operation, DNA polymerase α activity started to increase and reached its maximum level (more than twice the control) at day 4. At day 7, the enzyme level had decreased to the control level. Pulse-labeling experiment using radioactive thymidine showed that the rate of DNA synthesis increased approximately 2.5-fold in the same pattern as that of DNA polymerase α The mitotic index in hepatocytes also increased 10-fold at day 4 and then decreased. The proliferation of liver cells induced by obstructive jaundice mimics the regeneration of partially hepatectomized liver, although the response was slightly delayed and the proliferation was transient.
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