Volume 51, Issue 9 pp. 815-822

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Detection and Phylogenetic Analysis of Norovirus in Corbicula fluminea in a Freshwater River in Japan

Mika Saitoh,

Mika Saitoh

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515 Japan

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Hirokazu Kimura,

Hirokazu Kimura

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

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Kunihisa Kozawa,

Kunihisa Kozawa

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

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Osamu Nishio,

Osamu Nishio

National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011 Japan

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Akira Shoji,

Corresponding Author

Akira Shoji

Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515 Japan

Address correspondence to Dr. Akira Shoji, Department of Biological Sciences, Faculty of Engineering, Gunma University, 1-5-1 Tenjincho, Kiryu, Gunma 376-8515, Japan. Fax: + 81-277-30-1443. E-mail: [email protected]Search for more papers by this author

Mika Saitoh,

Mika Saitoh

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515 Japan

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Hirokazu Kimura,

Hirokazu Kimura

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

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Kunihisa Kozawa,

Kunihisa Kozawa

Gunma Prefectural Institute of Public Health and Environmental Sciences, Maebashi, Gunma, 371-0052 Japan

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Osamu Nishio,

Osamu Nishio

National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011 Japan

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Akira Shoji,

Corresponding Author

Akira Shoji

Department of Biological Sciences, Faculty of Engineering, Gunma University, Kiryu, Gunma, 376-8515 Japan

First published: 14 November 2013

https://doi.org/10.1111/j.1348-0421.2007.tb03977.x

Citations: 14

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Abstract

To study the molecular epidemiology of noroviruses (NoVs) in bivalves residing in freshwater rivers, we detected, quantified and phylogenetically analyzed the NoV genome in purified concentrates obtained from the gills and digestive diverticula of Corbicula fluminea in a freshwater river in Gunma Prefecture, Japan. We detected the NoV genome in 35 of the 58 C. fluminea samples. Based on our phylogenetic analysis, the NoV genome detected in the samples was classified into 4 genotypes (GI/1, GI/2, GI/3 and GI/4) in genogroup I and 5 genotypes (GII/3, GII/4, GII/5, GII/8 and GII/12) in genogroup II. The phylogenetic tree showed wide genetic diversity among the genogroups. In addition, more than 10⁴ copies of the NoV genome were detected in 2 of 35 samples. These results suggest that the freshwater bivalve C. fluminea is a reservoir for NoVs, similar to seawater bivalves such as oysters.

Abbreviations

GI: genogroup I
GII: genogroup II
N-J: neighbor-joining
NoV: norovirus
OTU: operational taxonomic unit
PBS: phosphate-buffered saline
PCR: polymerase chain reaction
RT-PCR: reverse transcription-PCR.

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Volume51, Issue9

September 2007

Pages 815-822

Detection and Phylogenetic Analysis of Norovirus in Corbicula fluminea in a Freshwater River in Japan

Abstract

Abbreviations

References

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Detection and Phylogenetic Analysis of Norovirus in Corbicula fluminea in a Freshwater River in Japan

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Abbreviations

References

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