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Intracellular Components Associated with Chikungunya Virus-Specific Ribonucleic Acids in Infected BHK21 Cells1

Corresponding Author

Akira Igarashi

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

Please address requests of reprints to Dr. Akira Igarashi, Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Yamada-kami, Suita-senri, Osaka 565, Japan.Search for more papers by this author

Fuyoko Sasao,

Fuyoko Sasao

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

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Konosuke Fukai,

Konosuke Fukai

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

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Akira Igarashi,

Corresponding Author

Akira Igarashi

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

Fuyoko Sasao,

Fuyoko Sasao

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

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Konosuke Fukai,

Konosuke Fukai

Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Suita

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First published: May 1973

https://doi.org/10.1111/j.1348-0421.1973.tb00791.x

Citations: 2

This work was presented at the 19th and 20th General Meetings of the Society of Japanese Virologists, held in Tokyo 1971 and in Osaka 1972, respectively.

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ABSTRACT

Cytoplasmic extracts of BHK21 cells infected with Chikungunya virus were analyzed by sucrose gradient sedimentation. Besides 140 S nucleocapsid, 65 S component associated with 26 S single-stranded ribonucleic acid was labeled with ³H-uridine in actinomycin-treated infected cells. The 65 S component, demonstrable in ethylenediaminetetraacetic acid-containing buffers, appeared to accumulate when protein synthesis was inhibited. After glutaraldehyde fixation, this component had a density of 1.48–1.50 g/ml in CsCl. Pulse-chase experiments did not indicate that the 65 S component was a precursor of the 140 S nucleocapsid.

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Volume17, Issue5

May 1973

Pages 401-408

Intracellular Components Associated with Chikungunya Virus-Specific Ribonucleic Acids in Infected BHK21 Cells1

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Intracellular Components Associated with Chikungunya Virus-Specific Ribonucleic Acids in Infected BHK21 Cells1

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