[Correction added on 24 July 2020, after first online publication: An error in the name of author Anna Benegiamo (mis-spelled ‘Bengiamo’ in the originally published version) has been corrected.]

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Italian Working Group on Diagnostic Hematology of the Italian Society of Clinical Chemistry, Clinical Molecular Biology (WGDH-SIBioC)

Maria Gioia,

Maria Gioia

Clinical Chemistry Laboratory, Abele Ajello Hospital, Mazara del Vallo, Italy

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Giorgio Da Rin,

Giorgio Da Rin

Medicine Laboratory, IRCCS Ospedale Policlinico San Martino, Genova, Italy

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Barbara Manenti,

Barbara Manenti

Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy

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Sarah Birindelli,

Sarah Birindelli

Clinical Pathology Unit, ASST Fatebenefratelli-Sacco, Milan, Italy

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Maria Laura Ciardelli,

Maria Laura Ciardelli

Clinical Chemistry Laboratory, Policlinico San Matteo-IRCCS, University of Pavia, Pavia, Italy

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Roberto Gentile,

Roberto Gentile

Clinical Chemistry Laboratory, Policlinico San Matteo-IRCCS, University of Pavia, Pavia, Italy

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Gianluca Beretta,

Gianluca Beretta

Clinical Chemistry Laboratory, Policlinico San Pietro - Istituti Ospedalieri Bergamaschi, San Pietro, Italy

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Giuseppe Lorusso,

Giuseppe Lorusso

Clinical Chemistry Laboratory, Policlinico San Pietro - Istituti Ospedalieri Bergamaschi, San Pietro, Italy

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Daniela Avino,

Daniela Avino

Hematological diagnostic Laboratory, P. O. “A. Tortora”, Pagani, Italy

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Anna Maria Di Fabio,

Anna Maria Di Fabio

Clinical Chemistry Laboratory, Avezzano Civic Hospital, Avezzano, Italy

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Francesco Dima,

Francesco Dima

Section of Clinical Biochemistry, University of Verona, Verona, Italy

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Alessandra Fanelli,

Alessandra Fanelli

Laboratory Department, Azienda Ospedaliera Careggi, Florence, Italy

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Maria Lorubbio,

Maria Lorubbio

Laboratory Department, Azienda Ospedaliera Careggi, Florence, Italy

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Sara Francione,

Sara Francione

Department of Clinical Chemistry and Microbiology, ASL Novara, Borgomanero, Italy

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Galina Marincheva,

Galina Marincheva

Department of Clinical Chemistry and Microbiology, ASL Novara, Borgomanero, Italy

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Alessandra Marini,

Alessandra Marini

Department of Clinical Chemistry, Versilia Hospital, Lido di Camaiore, Italy

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Angela Papa,

Angela Papa

Laboratory Medicine, Fondazione G. Monasterio CNR Regione Toscana, Pisa, Italy

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Elena Giannelli,

Elena Giannelli

Laboratory Medicine, Fondazione G. Monasterio CNR Regione Toscana, Pisa, Italy

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Rachele Pajola,

Rachele Pajola

Clinical Chemistry Laboratory, Ospedali Riuniti Padova Sud Schiavonia, Italy

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Andrea Panzeri,

Andrea Panzeri

Clinical Pathology Unit, ASST Fatebenefratelli-Sacco, Milan, Italy

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Silvia Pipitone,

Silvia Pipitone

Clinical Chemistry and Hematology Laboratory, University Hospital of Parma, Parma, Italy

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Anna Benegiamo,

Anna Benegiamo

Clinical Chemistry and Hematology Laboratory, University Hospital of Parma, Parma, Italy

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Roberta Rolla,

Roberta Rolla

Clinical Chemistry Laboratory, Department of Health Sciences, Maggiore Carità Hospital, University of Eastern Piedmont, Novara, Italy

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Matteo Vidali,

Matteo Vidali

Clinical Chemistry Laboratory, Department of Health Sciences, Maggiore Carità Hospital, University of Eastern Piedmont, Novara, Italy

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Sabrina Buoro,

Corresponding Author

Sabrina Buoro

[email protected]

orcid.org/0000-0001-7637-0727

Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy

Correspondence

Sabrina Buoro, Clinical Chemistry Laboratory - Papa Giovanni XXIII Hospital, Piazza OMS, 1, Bergamo — 24127, Italy.

Email: [email protected]

[Correction added on 24 July 2020, after first online publication: An error in the name of author Anna Benegiamo (mis-spelled ‘Bengiamo’ in the originally published version) has been corrected.]

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Italian Working Group on Diagnostic Hematology of the Italian Society of Clinical Chemistry, Clinical Molecular Biology (WGDH-SIBioC)

First published: 18 April 2020

https://doi.org/10.1111/ijlh.13204

Citations: 9

[Correction added on 20 July 2020, after first online publication: An error in the name of author Anna Benegiamo (mis-spelled ‘Bengiamo’ in the originally published version) has been corrected.]

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Abstract

Introduction

The correctness of the results of automated platelet analysis is still highly debated. The aim of this multicenter study, conducted according to international guidelines, was to verify the analytical performance of nine different types of hematology analyzers (HAs) in the automated platelet analysis.

Methods

Four hundred eighty-six peripheral blood samples (PB), collected in K₃EDTA tubes, were analyzed by ABX Pentra, ADVIA2120i, BC-6800, BC-6800 Plus, Cell-DYN Sapphire, DxH800, XE-2100, XE-5000, XN-20 with PLT-F App. Within-run imprecision and between-run imprecision were carried out using PB and material control, respectively. The carryover, low limit of quantification (LoQ), and the PB stability were evaluated.

Results

The carryover was absent for all HAs. The LoQ of PLT ranged between 2.0 (Cell-Dyn Sapphire) and 25.0 × 10⁹/L (ADVIA 2120i), while immature platelet fraction (IPF) ranged between 1.0 (XN-20) and 12.0 × 10⁹/L (XE-5000). The imprecision (%CV) increases as the platelet count decreases. No HAs showed desirable CV_APS for PLT counts less than 50.0 × 10⁹/L, with the exception of Cell-DYN Sapphire (CV 3.0% with PLT-O mean value of 26.7 × 10⁹/L), XN-20 (CV 2.4% with PLT-F mean value of 21.5 × 10⁹/L), and BC-6800 Plus (CV 1.9% with PLT-O mean value of 26.5 × 10⁹/L). The sample stability ranged between under two hours for MPV by ADVIA2120i and 8 hours for other PLT parameters and HAs.

Conclusion

The findings of this study may provide useful information regarding carryover, precision, and stability of platelet counts and parameters, especially in thrombocytopenic samples. Moreover, the stability of sample for platelet analysis is conditioned by the HA and by temperature and storage time.

CONFLICT OF INTEREST

The Authors stated that there are no conflicts of interest regarding the publication of this article.

Supporting Information

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Volume42, Issue5

October 2020

Pages 552-564

Multicenter evaluation of analytical performances of platelet counts and platelet parameters: Carryover, precision, and stability

Abstract

Introduction

Methods

Results

Conclusion

CONFLICT OF INTEREST

Supporting Information

REFERENCES

Citing Literature

References

Information

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Multicenter evaluation of analytical performances of platelet counts and platelet parameters: Carryover, precision, and stability

Abstract

Introduction

Methods

Results

Conclusion

CONFLICT OF INTEREST

Supporting Information

REFERENCES

Citing Literature

References

Related

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