iAMP21 screening by MLPA assay: Need for identification of characteristic pattern of amplification in addition to mean probe ratios to avoid false positivity
Anju Vijayan
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorMinu Singh
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorDeepak Bansal
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorSreejesh Sreedharanunni
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorNeelam Varma
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorMan Updesh Singh Sachdeva
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorCorresponding Author
Prateek Bhatia
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Correspondence
Prateek Bhatia, Paediatric Haematology-Oncology Unit, Advanced Paediatric Centre, Post Graduate Institute of Medical Education and Research, Chandigarh-160012, India.
Email: [email protected]
Search for more papers by this authorAnju Vijayan
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorMinu Singh
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorDeepak Bansal
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorSreejesh Sreedharanunni
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorNeelam Varma
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorMan Updesh Singh Sachdeva
Department of Haematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Search for more papers by this authorCorresponding Author
Prateek Bhatia
Paediatric Haematology-Oncology unit, Post Graduate Institute of Medical Education and Research, Chandigarh, India
Correspondence
Prateek Bhatia, Paediatric Haematology-Oncology Unit, Advanced Paediatric Centre, Post Graduate Institute of Medical Education and Research, Chandigarh-160012, India.
Email: [email protected]
Search for more papers by this author
REFERENCES
- 1Pui C-H, Yang JJ, Hunger SP, et al. Childhood acute lymphoblastic leukemia: progress through collaboration. J Clin Oncol. 2015; 33: 2938-2948.
- 2Strefford JC, van Delft FW, Robinson HM, et al. Complex genomic alterations and gene expression in acute lymphoblastic leukemia with intrachromosomal amplification of chromosome 21. Proc Natl Acad Sci USA. 2006; 103: 8167-8172.
- 3Rand V, Parker H, Russell LJ, et al. Genomic characterization implicates iAMP21 as a likely primary genetic event in childhood B-cell precursor acute lymphoblastic leukemia. Blood. 2011; 117: 6848-6855.
- 4Li Y, Schwab C, Ryan SL, et al. Constitutional and somatic rearrangement of chromosome 21 in acute lymphoblastic leukaemia. Nature. 2014; 508: 98-102.
- 5Moorman AV, Richards SM, Robinson HM, et al. Prognosis of children with acute lymphoblastic leukemia (ALL) and intrachromosomal amplification of chromosome 21 (iAMP21). Blood. 2007; 109: 2327-2330.
- 6Attarbaschi A, Mann G, Panzer-Grümayer R, et al. Minimal residual disease values discriminate between low and high relapse risk in children with B-cell precursor acute lymphoblastic leukemia and an intrachromosomal amplification of chromosome 21: the Austrian and German acute lymphoblastic leukemia Berlin-Frankfurt-Munster (ALL-BFM) trials. J Clin Oncol. 2008; 26: 3046-3050.
- 7Fuka G, Farias-Vieira TM, Hummel L, et al. Evaluation of multiplex ligation dependent probe amplification (MLPA) for identification of acute lymphoblastic leukemia with an intrachromosomal amplification of chromosome 21 (iAMP21) in a Brazilian population. Mol Cytogenet. 2015; 8: 35.
- 8Kim J, Lyu CJ, Shin S, et al. Frequency and clinical characteristics of intrachromosomal amplification of chromosome 21 in Korean childhood B-lineage acute lymphoblastic leukemia. Ann Lab Med. 2016; 36: 475-480.
- 9Harrison CJ. Blood Spotlight on iAMP21 acute lymphoblastic leukemia (ALL), a high-risk pediatric disease. Blood. 2015; 125: 1383-1386.
- 10Heerema NA, Carroll AJ, Devidas M, et al. Intrachromosomal amplification of chromosome 21 is associated with inferior outcomes in children with acute lymphoblastic leukemia treated in contemporary standard-risk children's oncology group studies: a report from the children's oncology group. J Clin Oncol. 2013; 31: 3397-3402.