Biocompatibility and bioactive potential of NeoPUTTY calcium silicate-based cement: An in vivo study in rats
Evelin Carine Alves Silva
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorJéssica Arielli Pradelli
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorGuilherme Ferreira da Silva
Department of Dentistry, Unisagrado, Bauru, São Paulo, Brazil
Search for more papers by this authorPaulo Sérgio Cerri
Department of Morphology, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Mario Tanomaru-Filho
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Correspondence
Mario Tanomaru-Filho, Department of Restorative Dentistry, Araraquara Dental School, São Paulo State, University-UNESP, Rua Humaitá, 1680, PO 331, CEP 14.801-903, Araraquara, SP, Brazil.
Email: [email protected]
Search for more papers by this authorJuliane Maria Guerreiro-Tanomaru
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorEvelin Carine Alves Silva
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorJéssica Arielli Pradelli
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorGuilherme Ferreira da Silva
Department of Dentistry, Unisagrado, Bauru, São Paulo, Brazil
Search for more papers by this authorPaulo Sérgio Cerri
Department of Morphology, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil
Search for more papers by this authorCorresponding Author
Mario Tanomaru-Filho
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Correspondence
Mario Tanomaru-Filho, Department of Restorative Dentistry, Araraquara Dental School, São Paulo State, University-UNESP, Rua Humaitá, 1680, PO 331, CEP 14.801-903, Araraquara, SP, Brazil.
Email: [email protected]
Search for more papers by this authorJuliane Maria Guerreiro-Tanomaru
Department of Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Araraquara, São Paulo, Brazil
Search for more papers by this authorAbstract
Aim
To evaluate the inflammatory reaction and the ability to induce mineralization activity of a new repair material, NeoPUTTY (NPutty; NuSmile, USA), in comparison with Bio-C Repair (BC; Angelus, Brazil) and MTA Repair HP (MTA HP; Angelus, Brazil).
Methodology
Polyethylene tubes were filled with materials or kept empty (control group, CG) and implanted in subcutaneous tissue of rats for 7, 15, 30, and 60 days (n = 6/group). Capsule thickness, number of inflammatory cells (ICs), fibroblasts, collagen content, and von Kossa analysis were performed. Unstained sections were evaluated under polarized light and by immunohistochemistry for osteocalcin (OCN). Data were submitted to two-way anova followed by Tukey's test (p ≤ .05), except for OCN. OCN data were submitted to Kruskal–Wallis and Dunn and Friedman post hoc tests followed by the Nemenyi test at a significance level of 5%.
Results
At 7, 15, and 30 days, thick capsules containing numerous ICs were seen around the materials. At 60 days, a moderate inflammatory reaction was observed for NPutty, BC while MTA HP presented thin capsules with moderate inflammatory cells. In all periods, NPutty specimens contained the highest values of ICs (p < .05). From 7 to 60 days, the number of ICs reduced significantly while an increase in the number of fibroblasts and birefringent collagen content was observed. At 7 and 15 days, no significant difference was observed in the immunoexpression of OCN (p > .05). At 30 and 60 days, NPutty showed the lowest values of OCN (p < .05). At 60 days, a similar immunoexpression was observed for BC and MTA HP (p > .05). In all time intervals, capsules around NPutty, BC, and MTA HP showed von Kossa-positive and birefringent structures.
Conclusions
Despite the greater inflammatory reaction promoted by NeoPutty than BC and MTA HP, the reduction in the thickness of capsules, the increase in the number of fibroblasts, and the reduction in the number of ICs indicate that this bioceramic material is biocompatible Furthermore, NeoPutty presents the ability to induce mineralization activity.
CONFLICT OF INTEREST STATEMENT
The authors have stated explicitly that there are no conflicts of interest in connection with this article.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.
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