Volume 64, Issue 7 pp. 921-934

Original Article

Paxillin and carbonic anhydrase IX are prognostic markers in gallbladder squamous cell/adenosquamous carcinomas and adenocarcinomas

Ziru Liu,

Ziru Liu

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

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Zhulin Yang,

Corresponding Author

Zhulin Yang

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

Address for correspondence: Z Yang, Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China. e-mail:[email protected]Search for more papers by this author

Song Jiang,

Song Jiang

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

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Qiong Zou,

Qiong Zou

Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, China

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Yuan Yuan,

Corresponding Author

Yuan Yuan

Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, China

Jinghe Li,

Jinghe Li

Department of Pathology, Basic School of Medicine, Changsha, China

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Daiqiang Li,

Daiqiang Li

Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, China

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Lufeng Liang,

Lufeng Liang

Department of Hepatobiliary and Pancreatic Surgery, Hunan Provincial People's Hospital, Changsha, China

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Meigui Chen,

Meigui Chen

Department of Pathology, Loudi Central Hospital, Loudi, China

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Senlin Chen,

Senlin Chen

Department of Pathology, Hunan Provincial Tumor Hospital, Changsha, China

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Ziru Liu,

Ziru Liu

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

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Zhulin Yang,

Corresponding Author

Zhulin Yang

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

Song Jiang,

Song Jiang

Research Laboratory of Hepatobiliary Diseases, Second Xiangya Hospital, Central South University, Changsha, China

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Qiong Zou,

Qiong Zou

Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, China

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Yuan Yuan,

Corresponding Author

Yuan Yuan

Department of Pathology, Third Xiangya Hospital, Central South University, Changsha, China

Jinghe Li,

Jinghe Li

Department of Pathology, Basic School of Medicine, Changsha, China

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Daiqiang Li,

Daiqiang Li

Department of Pathology, Second Xiangya Hospital, Central South University, Changsha, China

Search for more papers by this author

Lufeng Liang,

Lufeng Liang

Department of Hepatobiliary and Pancreatic Surgery, Hunan Provincial People's Hospital, Changsha, China

Search for more papers by this author

Meigui Chen,

Meigui Chen

Department of Pathology, Loudi Central Hospital, Loudi, China

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Senlin Chen,

Senlin Chen

Department of Pathology, Hunan Provincial Tumor Hospital, Changsha, China

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First published: 06 December 2013

https://doi.org/10.1111/his.12341

Citations: 13

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Abstract

Aims

Squamous cell/adenosquamous carcinomas (SC/ASC) are rare subtypes of gallbladder cancers (GBCs). Clinical characteristics of SC/ASC have not been well documented, and no biological markers of GBC carcinogenesis, progression and prognosis are available.

Methods and results

We measured paxillin and CAIX expression in 46 SC/ASCs and 80 adenocarcinomas (ACs) with immunohistochemistry and correlated these data with clinicopathological characteristics. Both paxillin expression and CAIX expression were associated significantly with larger tumours, a higher tumour-node-metastasis (TNM) stage, lymph node metastasis and invasiveness of SC/ASC and AC. Univariate Kaplan–Meier analysis confirmed that paxillin and CAIX expression were associated closely with decreased overall survival in SC/ASC (both P < 0.001) and AC (both P < 0.001). Multivariate Cox regression analysis confirmed that paxillin expression and CAIX expression both independently predicted poor prognosis in SC/ASC and AC patients. We also noted correlations with survival and tumour differentiation, tumour size, TNM stage, lymph node metastasis, tumour invasiveness and sample procurement methods.

Conclusions

Paxillin expression and CAIX expression are both related to clinical/biological behaviour and poor prognosis of GBC.

References

1Jemal A, Siegel R, Ward E et al. Cancer statistics, 2008. CA Cancer J. Clin. 2008; 58; 71–96.
10.3322/CA.2007.0010
CAS PubMed Web of Science® Google Scholar
2Jayaraman S, Jarnagin WR. Management of gallbladder cancer. Gastroenterol. Clin. North Am. 2010; 39; 331–342.
10.1016/j.gtc.2010.02.006
PubMed Web of Science® Google Scholar
3Reid KM, Ramos-De la Medina A, Donohue JHDiagnosis and surgical management of gallbladder cancer: a review. J. Gastrointest. Surg. 2007; 11; 671–681.
10.1007/s11605-006-0075-x
PubMed Web of Science® Google Scholar
4Hawkins WG, DeMatteo RP, Jarnagin WR et al. Jaundice predicts advanced disease and early mortality in patients with gallbladder cancer. Ann. Surg. Oncol. 2004; 11; 310–315.
10.1245/ASO.2004.03.011
PubMed Web of Science® Google Scholar
5Ootani T, Shirai Y, Tsukada K, Muto T. Relationship between gallbladder carcinoma and the segmental type of adenomyomatosis of the gallbladder. Cancer 1992; 69; 2647–2652.
10.1002/1097-0142(19920601)69:11<2647::AID-CNCR2820691105>3.0.CO;2-0
CAS PubMed Web of Science® Google Scholar
6Roa JC, Tapia O, Cakir A et al. Squamous cell and adenosquamous carcinomas of the gallbladder: clinicopathological analysis of 34 cases identified in 606 carcinomas. Mod. Pathol. 2011; 24; 1069–1078.
10.1038/modpathol.2011.68
PubMed Web of Science® Google Scholar
7Park SB, Kim YH, Rho HL, Chae GB, Hong SK. Primary carcinosarcoma of the gallbladder. J. Korean Surg. Soc. 2012; 82; 54–58.
10.4174/jkss.2012.82.1.54
Web of Science® Google Scholar
8Liu DC, Yang ZL. MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma. Diagn. Cytopathol. 2013; 41; 199–205.
10.1002/dc.21821
CAS PubMed Web of Science® Google Scholar
9Ivanov S, Liao SY, Ivanova A et al. Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrase in human cancer. Am. J. Pathol. 2001; 158; 905–919.
10.1016/S0002-9440(10)64038-2
CAS PubMed Web of Science® Google Scholar
10Madan R, Smolkin MB, Cocker R et al. Focal adhesion proteins as markers of malignant transformation and prognostic indicators in breast carcinoma. Hum. Pathol. 2006; 37; 9–15.
10.1016/j.humpath.2005.09.024
CAS PubMed Web of Science® Google Scholar
11Jaqadeeswaran R, Surawska H, Krishnaswamy J et al. Paxillin is a target for somatic mutations in lung cancer: implications for cell growth and invasion. Cancer Res. 2008; 68; 132–142.
10.1158/0008-5472.CAN-07-1998
CAS PubMed Web of Science® Google Scholar
12Zhao Y, Zhang X, Guda K et al. Identification and functional characterization of paxillin as a target of protein tyrosine phosphatase receptor T. Proc. Natl Acad. Sci. USA 2010; 107; 2592–2597.
10.1073/pnas.0914884107
CAS PubMed Web of Science® Google Scholar
13Mari L, Juha S, Tuomo J et al. Carbonic anhydrase isozymes IX and XII in gastric tumours. World J. Gastroenterol. 2003; 9; 1389–1401.
Google Scholar
14Span PN, Bussink J, Manders P. Carbonic anhydrase IX expression levels and prognosis in human breast cancer: association with treatment outcome. Br. J. Cancer 2003; 89; 271–276.
10.1038/sj.bjc.6601122
CAS PubMed Web of Science® Google Scholar
15Liao SY, Darcy KM, Randall LM et al. Prognostic relevance of carbonic anhydrase-IX in high-risk, early-stage cervical cancer: a Gynecologic Oncology Group study. Gynecol. Oncol. 2010; 116; 452–458.
10.1016/j.ygyno.2009.10.062
CAS PubMed Web of Science® Google Scholar
16Bai XM, Zhang W, Liu NB et al. Focal adhesion kinase: important to prostaglandin E2-mediated adhesion, migration and invasion in hepatocellular carcinoma cells. Oncol. Rep. 2009; 21; 129–136.
CAS PubMed Web of Science® Google Scholar
17Eetalli D, Lovat F, Tripodi F et al. The insulin-like growth factor receptor I promotes motility and invasion of bladder cancer cells through Akt- and mitogen-activated protein kinase-dependent activation of paxillin. Am. J. Pathol. 2010; 176; 2997–3006.
10.2353/ajpath.2010.090904
CAS Web of Science® Google Scholar
18Sanders AJ, Parr C, Martin TA et al. Genetic upregulation of matriptase-2 reduces the aggressiveness of prostate cancer cells in vitro and in vivo and affects FAK and paxillin localisation. J. Cell. Physiol. 2008; 216; 780–789.
10.1002/jcp.21460
CAS PubMed Web of Science® Google Scholar
19Ivanoc SV, Kuzmin I, Wie MH et al. Down-regulation of transmembrane carbonic anhydrase in renal carcinoma cell lines by wild-type von Hippel-Lindau transgene. Proc. Natl Acad. Sci. USA 1998; 95; 12596–12601.
10.1073/pnas.95.21.12596
PubMed Web of Science® Google Scholar
20Svastová E, Zilka N, Zat'ovicová M et al. Carbonic anhydrase IX reduces E-cadherin-mediated adhesion of MDCK cells via interaction with β-catenin. Exp. Cell Res. 2003; 290; 332–345.
10.1016/S0014-4827(03)00351-3
CAS PubMed Web of Science® Google Scholar
21Jain RK. Transport of molecules, particles, and cells in solid tumours. Annu. Rev. Biomed. Eng. 1999; 1; 241–321.
10.1146/annurev.bioeng.1.1.241
CAS PubMed Web of Science® Google Scholar
22Nishihara K, Nagai E, Izumi Y, Yamaguchi K, Tsuneyoshi M. Adenosquamous carcinoma of the gallbladder: a clinicopathological, immunohistochemical and flow-cytometric study of twenty cases. Jpn. J. Cancer Res. 1994; 85; 389–399.
10.1111/j.1349-7006.1994.tb02372.x
CAS PubMed Web of Science® Google Scholar
23Kondo M, Dono K, Sakon M et al. Adenosquamous carcinoma of the gallbladder. Hepatogastroenterology 2002; 49; 1230–1234
PubMed Web of Science® Google Scholar

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Volume64, Issue7

June 2014

Pages 921-934

Paxillin and carbonic anhydrase IX are prognostic markers in gallbladder squamous cell/adenosquamous carcinomas and adenocarcinomas

Abstract

Aims

Methods and results

Conclusions

References

Citing Literature

References

Information

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Paxillin and carbonic anhydrase IX are prognostic markers in gallbladder squamous cell/adenosquamous carcinomas and adenocarcinomas

Abstract

Aims

Methods and results

Conclusions

References

Citing Literature

References

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