Human biliary tree stem/progenitor cells immunomodulation: Role of hepatocyte growth factor
Corresponding Author
Tullia Maraldi
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Correspondence: Dr Tullia Maraldi, Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. Email: [email protected]Search for more papers by this authorMarianna Guida
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
EURAC Research, Center for Biomedicine, Bolzano, Italy
Search for more papers by this authorFrancesca Beretti
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorElisa Resca
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorGuido Carpino
Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorVincenzo Cardinale
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorRaffaele Gentile
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAndrea Ardizzoni
Department of Diagnostics, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorAlba Murgia
Department of Medical and Surgical Sciences for Children & Adults University-Hospital of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorDomenico Alvaro
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Department of Movement, Human and Health Sciences, Division of Health Sciences, University of Rome “Foro Italico”, Rome, Italy
Search for more papers by this authorEugenio Gaudio
Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAnto De Pol
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorCorresponding Author
Tullia Maraldi
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Correspondence: Dr Tullia Maraldi, Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, via del Pozzo 71, 41124, Modena, Italy. Email: [email protected]Search for more papers by this authorMarianna Guida
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
EURAC Research, Center for Biomedicine, Bolzano, Italy
Search for more papers by this authorFrancesca Beretti
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorElisa Resca
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorGuido Carpino
Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorVincenzo Cardinale
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorRaffaele Gentile
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAndrea Ardizzoni
Department of Diagnostics, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorAlba Murgia
Department of Medical and Surgical Sciences for Children & Adults University-Hospital of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorDomenico Alvaro
Department of Medico-Surgical Sciences and Biotechnologies, Polo Pontino, Sapienza University of Rome, Rome, Italy
Department of Movement, Human and Health Sciences, Division of Health Sciences, University of Rome “Foro Italico”, Rome, Italy
Search for more papers by this authorEugenio Gaudio
Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy
Search for more papers by this authorAnto De Pol
Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy
Search for more papers by this authorAbstract
Aim
Human biliary tree stem/progenitor cells (hBTSC) are multipotent epithelial stem cells with the potential for allogenic transplant in liver, biliary tree, and pancreatic diseases. Human mesenchymal stem cells, but also epithelial stem cells, are able to modulate immune responses with different types of secretion molecules.
Methods
The initial aim of the present study was to develop for the first time a culture protocol in order to expand hBTSC in vitro through passages, allowing to maintain a similar stem cell and secretome profile. Furthermore, we investigated the secretome profile of the hBTSC to assess the production of molecules capable of affecting immune feedback.
Results
We found that hepatocyte growth factor produced by hBTSC exerts its cytoprotective role inducing apoptosis in human immune cells, such as lymphocytes.
Conclusions
The present study, therefore, supports the hypothesis that hBTSC can be useful for the purpose of regenerative medicine, as they can be banked and expanded, and they can secrete immunoregulatory factors.
Supporting Information
Oncogenic activity evaluation
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