Genotypic variation of cell wall composition and its conversion efficiency in Miscanthus sinensis, a potential biomass feedstock crop in China
Hua Zhao
College of plant science & technology of Huazhong Agricultural University, Wuhan, 430070 China
Search for more papers by this authorQian Li
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190 China
Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorJunrong He
Institute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu, 610066 China
Search for more papers by this authorJiangyan Yu
Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorJunpin Yang
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066 China
Search for more papers by this authorChunzhao Liu
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorCorresponding Author
Junhua Peng
College of plant science & technology of Huazhong Agricultural University, Wuhan, 430070 China
Correspondence: Dr. Junhua Peng, tel: +86 10 582 907 79, fax: +86 10 582 908 08, e-mail: [email protected]Search for more papers by this authorHua Zhao
College of plant science & technology of Huazhong Agricultural University, Wuhan, 430070 China
Search for more papers by this authorQian Li
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190 China
Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorJunrong He
Institute of Horticulture, Sichuan Academy of Agricultural Sciences, Chengdu, 610066 China
Search for more papers by this authorJiangyan Yu
Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049 China
Search for more papers by this authorJunpin Yang
Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066 China
Search for more papers by this authorChunzhao Liu
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190 China
Search for more papers by this authorCorresponding Author
Junhua Peng
College of plant science & technology of Huazhong Agricultural University, Wuhan, 430070 China
Correspondence: Dr. Junhua Peng, tel: +86 10 582 907 79, fax: +86 10 582 908 08, e-mail: [email protected]Search for more papers by this authorAbstract
Plant cell walls are composed of cellulose microfibrils embedded in a cross-linked-net of matrix polysaccharides and co-polymerized with lignin. The study presented the genotypic variations of cell wall composition, biohydrogen production, and lignocellulose degradation ratio in a collection of 102 Miscanthus sinensis (M. Sinensis, hereafter) accessions collected from a wide geographical range in China. Significant variations were observed for the determined traits, cellulose content, hemicellulose content, cellulose and hemicellulose degradation efficiency, and biohydrogen yield. The cellulose, hemicellulose, and lignin contents ranged from 30.20–44.25, 28.97–42.65, and 6.96–20.75%, respectively. The degradation ratio of cellulose and hemicellulose varied from 2.08% to 37.87% and from 14.71% to 52.50%, respectively. The feedstock was fermented to produce biohydrogen, and the production varied from 14.59 to 40.66 ml per gram of Miscanthus biomass. The expression profile of three cellulose synthase (MsCesA) genes was initially established to indicate the genotypic difference among the M. sinensis accessions. Pearson's correlations were conducted to reveal the perplexing relationship between the tested traits, biohydrogen yield, cell wall composition and their degradation efficiency. In addition, the relationship pattern, between the test traits and the geographic factors corresponding with the original place, was investigated. The result showed that the significant variation among the M. sinensis genotypes is the result of natural selection in different environments of their original habitats. Improvement in cell wall composition and structure and enhancement of lignocellulose degradation ratio could significantly increase sustainable bioenergy production.
Supporting Information
| Filename | Description |
|---|---|
| gcbb12115-sup-0001-TableS1-S2.docWord document, 208 KB | Table S1. Geographical parameters of the original habitat and determined bioenergy traits of 102 M. sinensis accessions. Table S2. Geographical parameters of the original habitat and determined bioenergy traits of six elite M. sinensis accessions. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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