The role of forkhead box class O1 during implant osseointegration
Feng Zhou
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorZumu Yi
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorYingying Wu
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorCorresponding Author
Yi Xiong
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Correspondence
Yi Xiong, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, 14, 3rd Section Renmin Nan Road, Chengdu, China.
Email: [email protected]
Search for more papers by this authorFeng Zhou
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorZumu Yi
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorYingying Wu
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Search for more papers by this authorCorresponding Author
Yi Xiong
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, China
Correspondence
Yi Xiong, Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, 14, 3rd Section Renmin Nan Road, Chengdu, China.
Email: [email protected]
Search for more papers by this authorAbstract
FOXO1, a member of the forkhead family of transcription factors, plays a vital role in the osteogenic lineage commitment of mesenchymal stem cells, and affects multiple cellular functions of osteogenic cells. However, prior studies have focused on mesenchymal stem cells but not on differentiated osteoblasts. In addition, studies about the role of FOXO1 during osseointegration are lacking. In this present study, we constructed osteoblast conditional FOXO1 knock-out mice and lentivirus-mediated FoxO1 overexpression to investigate maxillary titanium implant osseointegration. After 4 wk post implant placement, micro-computed tomography, histomorphometric analyses, and RT-qPCR assays were performed. Results showed that compared with the control group, overexpression of FOXO1 significantly enhanced bone formation around implant and bone-implant contact ratio, while loss of FOXO1 impaired peri-implant osteogenesis and osseointegration. Moreover, overexpression of FoxO1 enhanced expression of osteogenesis-related genes, such as Runx2, Alp1, Col1a1, and Bglap. Whereas, knock-out of Foxo1 reduced the expression of osteogenesis-related genes. Taken together, our results suggested that FOXO1 in osteoblasts could enhance osteogenesis-related gene expression to improve osseointegration.
CONFLICT OF INTEREST
The authors declare that they have no competing interests that may inappropriately influence their work.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available on request from the corresponding author.
Supporting Information
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