Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats
Correction(s) for this article
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Corrigendum
- Volume 129Issue 6European Journal of Oral Sciences
- First Published online: December 2, 2021
Yan-Hui Wu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorQing-Ting Yao
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorShao-Hua Liu
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorXiao-Bin Song
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorAi-Pi-Zi-Gu-Li Yakupu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorLi-Dan Lu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorLiang Shi
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorCorresponding Author
Hui Xu
Stomatology School of Xinjiang Medical University, Urumqi, China
Institute of Stomatology, Shandong University, Jinan, China
Correspondence
Hui Xu, Stomatology School of Xinjiang Medical University, Urumqi, 830001, China.
Email: [email protected]
Search for more papers by this authorYan-Hui Wu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorQing-Ting Yao
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorShao-Hua Liu
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorXiao-Bin Song
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorAi-Pi-Zi-Gu-Li Yakupu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorLi-Dan Lu
Stomatology School of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China
Search for more papers by this authorLiang Shi
Postgraduate College of Xinjiang Medical University, Urumqi, China
Department of Oral and Maxillofacial Surgery, Qilu Hospital of Shandong University, Jinan, China
Search for more papers by this authorCorresponding Author
Hui Xu
Stomatology School of Xinjiang Medical University, Urumqi, China
Institute of Stomatology, Shandong University, Jinan, China
Correspondence
Hui Xu, Stomatology School of Xinjiang Medical University, Urumqi, 830001, China.
Email: [email protected]
Search for more papers by this authorFunding Information
This study was supported by grants from the Key Research and Development Program of Shandong Province (GG201709220011) and the Shandong Provincial Natural Science Foundation (ZR2020MH187).
Abstract
To investigate the effects of radiation on rat submandibular glands and the possible protective effects of ischemic preconditioning, the submandibular glands of Wistar rats were subjected to in situ radiation after ischemic preconditioning. The glands were exposed to X-radiation at a single dose of 20 Gy. Ischemic preconditioning was achieved by three min of ischemia and three min of reperfusion, repeated three times before irradiation. Salivary secretion, histological changes, alterations in tight junctions, and the levels of oxidative stress, pro-inflammatory cytokines, and water secretion proteins mediated by the muscarinic acetylcholine M3 subtype receptor were determined at 1 and 12 weeks post-irradiation. In glands subjected to irradiation only, the secretion, superoxide dismutase activity, tight junction width, acinar cell number, and M3 receptor and aquaporin-5 levels were lower at 1 and 12 weeks than seen in the ischemically preconditioned irradiated glands. In contrast, tumor necrosis factor-α, malondialdehyde, myeloperoxidase activity, and the expression of the tight junction protein claudin-4 were significantly higher in the irradiated only glands. Our study revealed that radiation caused a series of injury-stress responses, especially damage to the water secretion pathway mediated by the M3 receptor that ultimately led to hyposecretion, which might play an important role in the dysfunction of the irradiated only glands. Ischemic preconditioning reduced the radiation-induced injury to submandibular glands and ameliorated salivary hyposecretion.
CONFLICT OF INTERESTS
The authors declare no conflicts of interest with respect to the authorship and/or publication of this article.
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