Characterization of the T cell response in allergic contact dermatitis caused by corticosteroids
Corresponding Author
Marie Baeck
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Marie Baeck, Department of Dermatology, Cliniques Universitaires Saint-Luc, U.C.L., Avenue Hippocrate 10, B-1200 Brussels, Belgium. Tel: +32 2 764 14 72; Fax: +32 2 764 80 27. E-mail: [email protected]Search for more papers by this authorAngèle Soria
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorLiliane Marot
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorIvan Theate
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorEmilie Hendrickx
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAstrid Van Belle
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAn Goossens
Department of Dermatology, University Hospital, Katholieke Universiteit Leuven, 3000 Leuven, Belgium
Search for more papers by this authorDominique Tennstedt
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorClaire Dachelet
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorJohnathan Jaeger
Department of Statistics, SMCS, IMMAQ Institute of Multidisciplinary Research for Quantitative Modelling and Analysis, Université Catholique de Louvain, 1348 Louvain-La-Neuve, Belgium
Search for more papers by this authorCharles Pilette
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Department of Pneumology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorJean-Christophe Renauld
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorNicolas Van Baren
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAurore Rozières
Department of Immuno-Allergology, University Hospital Centre Lyon Sud, Hospices Civiles de Lyon, 69495 Pierre-Benite, France
Search for more papers by this authorJean-Fançois Nicolas
Department of Immuno-Allergology, University Hospital Centre Lyon Sud, Hospices Civiles de Lyon, 69495 Pierre-Benite, France
Search for more papers by this authorLaure Dumoutier
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorCorresponding Author
Marie Baeck
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Marie Baeck, Department of Dermatology, Cliniques Universitaires Saint-Luc, U.C.L., Avenue Hippocrate 10, B-1200 Brussels, Belgium. Tel: +32 2 764 14 72; Fax: +32 2 764 80 27. E-mail: [email protected]Search for more papers by this authorAngèle Soria
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorLiliane Marot
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorIvan Theate
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorEmilie Hendrickx
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAstrid Van Belle
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAn Goossens
Department of Dermatology, University Hospital, Katholieke Universiteit Leuven, 3000 Leuven, Belgium
Search for more papers by this authorDominique Tennstedt
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorClaire Dachelet
Department of Dermatology, Cliniques Universitaires Saint-Luc, Universite’ Catholique de Louvain, 1200 Brussels, Belgium
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorJohnathan Jaeger
Department of Statistics, SMCS, IMMAQ Institute of Multidisciplinary Research for Quantitative Modelling and Analysis, Université Catholique de Louvain, 1348 Louvain-La-Neuve, Belgium
Search for more papers by this authorCharles Pilette
Pôle Pneumologie, ORL et Dermatologie Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain, 1200 Brussels, Belgium
Department of Pneumology, Cliniques Universitaires Saint-Luc, Université Catholique de Louvain, 1200 Brussels, Belgium
Search for more papers by this authorJean-Christophe Renauld
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorNicolas Van Baren
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorAurore Rozières
Department of Immuno-Allergology, University Hospital Centre Lyon Sud, Hospices Civiles de Lyon, 69495 Pierre-Benite, France
Search for more papers by this authorJean-Fançois Nicolas
Department of Immuno-Allergology, University Hospital Centre Lyon Sud, Hospices Civiles de Lyon, 69495 Pierre-Benite, France
Search for more papers by this authorLaure Dumoutier
Ludwig Institute for Cancer Research, B-1200 Brussels, Belgium
de Duve Institute, Université Catholique de Louvain, B-1200 Brussels, Belgium
Search for more papers by this authorConflicts of interest: The authors have declared no conflicts.
Summary
Background Delayed allergic hypersensitivity reactions have classically been described as type IV reactions, which are caused by T cells; however, the respective roles of CD4+ and CD8+ cells are yet to be defined. A central role for CD8+ cytotoxic T cells as effector cells has been suggested.
Objectives To determine the type of T cell involved in corticosteroid allergy.
Methods We analysed the kinetics of T cell recruitment and the cytokine production profile in positive patch tests of 27 corticosteroid-sensitized patients, as compared with control sites and control subjects. Skin biopsies, collected at 8, 24 and 48 hr following drug application, were embedded in paraffin for histological and immunohistological staining, and, in some cases, also deep-frozen for gene expression analyses.
Results CD3+ T cells were rapidly recruited in concert with the positivity of the patch test sites. High levels of interleukin (IL)-4, IL-5 and, to a lesser extent, interferon-γ suggested that both Th2 and Th1 cytokines were implicated. IL-4 was also produced by γδ T cell receptor (TCR) lymphocytes.
Conclusions This study showed that, in allergic contact dermatitis caused by corticosteroids, the inflammatory infiltrate is composed of CD3+ T cells with a predominant Th2 cytokine profile, among which IL-4 is also produced by γδ TCR lymphocytes.
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