Comparative evaluation of cell adhesion and proliferation on surface treated titanium discs with a Ch-Mr-Tr hydrogelíí-an in vitro study

Background

Inadequate bone to implant contact and increased time for osseointegration being a major concern in implant based rehabilitation many efforts were made to increase the proliferation of osteoblasts and matrix laying down potent. In this study, we modify the surface of implants through an organic coating in the form of CHITOSAN-MORINGA OLEIFERA-TRIGONELLA hydrogel proliferating osteoblasts so as to achieve an intimate bone-implant contact.

Aim/Hypothesis

The aim of this study is to evaluate and compare adhesion, proliferation of MG-63 osteoblast like cells in response to chitosan-moringa-trigonella hydrogels on titanium discs. To evaluate the proliferative nature of MG-63 osteoblast like cells after subjecting it onto novel

Material and Methods

Preparation of CH-MR- CH (3 gm in 100 ml) was dissolved in diluted acetic acid and a pH was adjusted to neutral using 0.1 N NAOH. MO was added with constant stirring.CH-FENUGREEK(FU) preparation- FU Extract was prepared with 99.5% ethanol by maceration technique (100 gm powder in 500 ml). The pure extract obtained was incorporated into CH. (CH-MR- FU) was prepared by mixing both the above mentioned in 50–50 by weight. Influence on human osteoblast like cell(MG63) was assessed in terms of cell adhesion viability. The cell viability was measured using MTT assay and matrix mineralization was assessed by von Kossa histochemical staining.

Results

Our results indicated that CH _MR- FU were taken up by the MG63 cells. The cell number on the Ti DISC with CS-MR-FU group significantly increased compared to control groups at 6, 12, and 24 hours (P < 0.05) with Higher osteoblast metabolic activity and mineralization than control. The proliferation index of osteoblasts was faster on TEST was more than on control as evidenced by the proliferation indices of 4.89 ± 0.03 and 2.42 ± 0.16, respectively.

Conclusion and Clinical Implications

The novel technique with surface treated Ti can efficiently enhance osteoblast seeding and migration onto the surface of the implant and increase subsequent cell-cell interactions and shorten the period of cell proliferation. This novel system is thought to be useful in the development of biological seal around the implant and contact osteogenesis and cell-based strategies for the repair or replacement of tissue and other novel therapies.