Influence of magnetized abutment on biological seal of the implant–soft tissue interface by reconstructed human gingiva (RHG) model

HGVPN ePOSTER CLINICAL RESEARCH – PERI-IMPLANT BIOLOGY

Background: osseointegration in implants have received a lot of attention. Recently, the focus has extended towards the development of abutment materials supporting gingival soft tissue attachment. The attachment of the soft tissue to the implant/abutment surface is necessary to form a biological seal that protects the pathogenic microbial colonization which can lead to periimplantitis and bone resorption culminating in dental implant failure.

Aim/Hypothesis: To evaluate the Influence of magnetized abutment surface on biological seal of the implant–soft tissue interface that would enable junctional epithelium(JE) attachment to the abutment through an internal basal lamina and hemidesmosomes in order to prevent the JE from migrating too in depth.

Materials and Methods: We developed novel magnetic nanoparticles (MNs) coated with chitosan(CS) (CS-MNs) for enhancing cellular migration using magnetic force. CS-MNs were characterized by XRD, UV, SEM. The CS-MNs where spherical shape with a diameter from 10 nm to 15 nm. The magnetized abutments surface was treated with CS-MNs. Influence of CS-MNs treated abutment on reconstructed human gingiva (RHG) model consisting of differentiated gingival epithelium cells was assessed in terms of Epithelial attachment, down-growth along the abutment surface, quantitative analysis by permeability and cell attachment tests. Phenotype via histomorphology, scanning electron microscopy, and immunohistochemistry 10 days after implantation.

Results: Abutment values obtained from the intraexperiment replicates including measurement of the left and right side of the abutment (total of four measurements) were first averaged, and Differences were determined using one-way ANOVA followed by Dunnett's uncorrected multiple comparisons test. Differences were considered significant at P < 0.05. histomorphometric analyses showed epithelial growth near perpendicular to the surface of abutments. The magnification clearly showed individual keratinocytes spread and attached to abutment surfaces via filopodia extensions.

Conclusions and Clinical Implications: The current RHG model shows the active migration of epithelial cells near perpendicularly along the magnetized abutment surface with higher gingival fibroblast attachment as compared with an untreated surface. This novel system is thought to be useful in the development of biological seal around the implant and cell-based strategies for the repair or replacement of tissue and other novel therapies.

Keywords: Soft tissue-implant interactions, biological seal, tissue-engineered oral mucosa, three-dimensional in vitro model