Cell-derived microparticles and sickle cell disease chronic vasculopathy in sub-Saharan Africa: A multinational study
Abdoul K. Dembélé
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Equal contributionSearch for more papers by this authorClaudine Lapoumeroulie
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Equal contributionSearch for more papers by this authorMor Diaw
Université Cheikh Anta Diop de Dakar (UCAD), Laboratoire de Physiologie et Explorations Fonctionnelles, Dakar, Sénégal
Unité Mixte Internationale (UMI 3189), ‘Environnement, Santé, Sociétés’ CNRS, UCAD, CNRST, USTTB, UGB, Dakar, Sénégal
Equal contributionSearch for more papers by this authorOumarou Tessougue
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Search for more papers by this authorLucile Offredo
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorDapa A. Diallo
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Search for more papers by this authorSaliou Diop
Centre National de Transfusion Sanguine (CNTS), Dakar, Sénégal
Search for more papers by this authorJacques Elion
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Search for more papers by this authorYves Colin-Aronovicz
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Search for more papers by this authorPierre-Louis Tharaux
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorXavier Jouven
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorMarc Romana
Laboratoire d’Excellence GR-Ex, Paris, France
Université des Antilles, UMR_S1134, BIGR, INSERM, Pointe-à-Pitre, France
Equal contributionSearch for more papers by this authorBrigitte Ranque
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Equal contributionSearch for more papers by this authorCorresponding Author
Caroline Le Van Kim
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Equal contributionCorrespondence: Caroline Le Van Kim, Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge, INSERM, F-75015 Paris, France; Institut National de Transfusion Sanguine, 6 rue Alexandre Cabanel, 75739 Paris, France.
E-mail: [email protected]
Search for more papers by this authorAbdoul K. Dembélé
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Equal contributionSearch for more papers by this authorClaudine Lapoumeroulie
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Equal contributionSearch for more papers by this authorMor Diaw
Université Cheikh Anta Diop de Dakar (UCAD), Laboratoire de Physiologie et Explorations Fonctionnelles, Dakar, Sénégal
Unité Mixte Internationale (UMI 3189), ‘Environnement, Santé, Sociétés’ CNRS, UCAD, CNRST, USTTB, UGB, Dakar, Sénégal
Equal contributionSearch for more papers by this authorOumarou Tessougue
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Search for more papers by this authorLucile Offredo
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorDapa A. Diallo
Centre de Recherche et de Lutte contre la Drépanocytose (CRLD), Bamako, Mali
Search for more papers by this authorSaliou Diop
Centre National de Transfusion Sanguine (CNTS), Dakar, Sénégal
Search for more papers by this authorJacques Elion
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Search for more papers by this authorYves Colin-Aronovicz
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Search for more papers by this authorPierre-Louis Tharaux
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorXavier Jouven
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Search for more papers by this authorMarc Romana
Laboratoire d’Excellence GR-Ex, Paris, France
Université des Antilles, UMR_S1134, BIGR, INSERM, Pointe-à-Pitre, France
Equal contributionSearch for more papers by this authorBrigitte Ranque
Laboratoire d’Excellence GR-Ex, Paris, France
Université de Paris, INSERM, Paris Cardiovascular Centre - PARCC, Paris, France
Equal contributionSearch for more papers by this authorCorresponding Author
Caroline Le Van Kim
Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge (BIGR), INSERM, Paris, France
Institut National de Transfusion Sanguine, Paris, France
Laboratoire d’Excellence GR-Ex, Paris, France
Equal contributionCorrespondence: Caroline Le Van Kim, Université de Paris, UMR_S1134, Biologie Intégrée du Globule Rouge, INSERM, F-75015 Paris, France; Institut National de Transfusion Sanguine, 6 rue Alexandre Cabanel, 75739 Paris, France.
E-mail: [email protected]
Search for more papers by this authorSummary
Although most individuals with sickle cell disease (SCD) live in sub-Saharan Africa, the natural history of the disease on this continent remains largely unknown. Intravascular haemolysis results in activation of circulating blood cells and release of microparticles (MPs) that exert pro-inflammatory effects and contribute to vascular damage. We designed a case-control study nested in the CADRE cohort (Coeur-Artère-DRÉpanocytose, clinical trials.gov identifier NCTO3114137) and based on extreme phenotypes, to analyse blood cell-derived MPs in 232 adult SS patients at steady state in Bamako and Dakar. Thirty-six healthy adult controls matched by age and sex were recruited in Bamako. The MPs concentrations were higher in SS patients compared to AA controls with a predominance of erythrocyte- and reticulocyte-derived MPs. These erythroid-derived MPs were significantly lower in patients with retinopathy (P = 0·022). Reticulocyte-derived MPs were significantly negatively and positively associated with a history of priapism (P = 0·020) and leg ulcers (P = 0·041) respectively. We describe for the first time the comparative patterns of plasma MPs in healthy subjects and
patients with SCD living in sub-Saharan Africa and exhibiting various complications. Because our present results show no clear pattern of correlation between erythroid MPs and the classical hyper-haemolytic complications, we hypothesise a weak relevance of the hyper-haemolysis versus hyper-viscous paradigm in Africa.
CONFLICT OF INTEREST
The authors have no conflict of interest to declare.
Supporting Information
| Filename | Description |
|---|---|
| bjh17242-sup-0001-FigS1.tiffTIFF image, 1.1 MB | Fig S1. Blood-cell-type origin of MPs in SS patients in Bamako (Mali) and Dakar (Senegal). |
| bjh17242-sup-0002-TableSI.pptxapplication/ms-powerpoint, 36.3 KB | Table SI. Specific antibodies used to determine the cellular origin of the MP. |
| bjh17242-sup-0003-TableSII.pptxapplication/ms-powerpoint, 39.8 KB | Table SII. Comparison of the clinical and haematological features of the SS patients in Bamako and Dakar. |
| bjh17242-sup-0004-TableSIII.pptxapplication/ms-powerpoint, 42.5 KB | Table SIII. Correlation between vascular complications and MPs concentration. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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