Mass cytometry identifies expansion of double positive and exhausted T cell subsets in the tumour microenvironment of patients with POEMS syndrome
Taxiarchis V. Kourelis
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorDragan Jevremovic
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorErik Jessen
Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorSurendra Dasari
Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorJose C. Villasboas
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorAngela Dispenzieri
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorShaji Kumar
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorTaxiarchis V. Kourelis
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorDragan Jevremovic
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorErik Jessen
Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorSurendra Dasari
Department of Health Sciences Research, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorJose C. Villasboas
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
Search for more papers by this authorAngela Dispenzieri
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorShaji Kumar
Division of Hematology, Department of Medicine, Mayo Clinic, Rochester, MN, USA
These authors contributed equally as senior authors.Search for more papers by this authorCorrespondence: Taxiarchis V. Kourelis, Division of Hematology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
E-mail: [email protected]
Summary
We sought to dissect the tumour microenvironment in a small cohort (N = 10) of patients with POEMS at diagnosis and after therapy using mass cytometry. We included 10 MGUS patients as controls. We identified 29 immune cell subsets in the CD45+ and CD3+ compartments. Double positive T cells and PD-1 positive CD4 T cells were expanded and naïve CD4 T cells were decreased in the bone marrow of patients with newly diagnosed/progressing POEMS. These findings provide evidence for possible antigenic-driven selection as a driver of disease pathogenesis in POEMS.
Conflict of interest
The authors have no competing financial interests in relation to the work described.
Supporting Information
| Filename | Description |
|---|---|
| bjh16522-sup-0001-FigS1.tifTIFF image, 294.8 KB |
Figure S1. Characterising the main immune components of the marrow microenvironment in common dysproteinemias. |
| bjh16522-sup-0002-FigS2.tifTIFF image, 507 KB |
Figure S2. A correlation matrix of all identified immune populations in newly diagnosed or relapsed POEMS (A) and in all POEMS patients included in the study (B). |
| bjh16522-sup-0003-FigS3.tifTIFF image, 109.5 KB |
Figure S3. Frequencies of the T2, T5 and T16 T cell subsets and CD163+ monocytes in patients with POEMS syndrome at diagnosis or progression (ND/PROG), at day +100 after autologous stem cell transplant (post ASCT) and at various time points after treatment with ixazomib, lenalidomide and dexamethasone (post IRD). |
| bjh16522-sup-0004-TableS1.docxWord document, 15 KB |
Table S1. Baseline characteristics of patients. |
| bjh16522-sup-0005-DataS1.docxWord document, 22.8 KB |
Data S1. Our antibody panel included 33 antibodies directed against well characterised surface markers. |
| bjh16522-sup-0006-Legends.docxWord document, 12.7 KB |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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