General Dermatology

Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

Corresponding Author

J.F. Schwensen

[email protected]

National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

Correspondence

Jakob F. Schwensen.

E-mail: [email protected]

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C. Menné Bonefeld,

C. Menné Bonefeld

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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C. Zachariae,

C. Zachariae

Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

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C. Agerbeck,

C. Agerbeck

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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T.H. Petersen,

T.H. Petersen

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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C. Geisler,

C. Geisler

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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U.E. Bollmann,

U.E. Bollmann

Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark

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K. Bester,

K. Bester

Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark

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J.D. Johansen,

J.D. Johansen

National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

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J.F. Schwensen,

Corresponding Author

J.F. Schwensen

[email protected]

National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

Correspondence

Jakob F. Schwensen.

E-mail: [email protected]

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C. Menné Bonefeld,

C. Menné Bonefeld

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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C. Zachariae,

C. Zachariae

Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

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C. Agerbeck,

C. Agerbeck

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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T.H. Petersen,

T.H. Petersen

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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C. Geisler,

C. Geisler

Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

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U.E. Bollmann,

U.E. Bollmann

Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark

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K. Bester,

K. Bester

Department of Environmental Science, Aarhus University, Frederiksborgvej 399, 4000 Roskilde, Denmark

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J.D. Johansen,

J.D. Johansen

National Allergy Research Centre, Department of Dermato-Allergology, Copenhagen University Hospital Gentofte, Kildegårds Allé, 2900 Hellerup, Denmark

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First published: 25 June 2016

https://doi.org/10.1111/bjd.14825

Citations: 44

Funding sources The National Allergy Research Centre is supported by a nonrestricted grant from the Danish Environmental Protection Agency. The Aage Bang Foundation, a nonprofit organization, additionally funded this work.

Conflicts of interest None declared.

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Summary

Background

In the light of the exceptionally high rates of contact allergy to the preservative methylisothiazolinone (MI), information about cross-reactivity between MI, octylisothiazolinone (OIT) and benzisothiazolinone (BIT) is needed.

Objectives

To study cross-reactivity between MI and OIT, and between MI and BIT.

Methods

Immune responses to MI, OIT and BIT were studied in vehicle and MI-sensitized female CBA mice by a modified local lymph node assay. The inflammatory response was measured by ear thickness, cell proliferation of CD4⁺ and CD8⁺ T cells, and CD19⁺ B cells in the auricular draining lymph nodes.

Results

MI induced significant, strong, concentration-dependent immune responses in the draining lymph nodes following a sensitization phase of three consecutive days. Groups of MI-sensitized mice were challenged on day 23 with 0·4% MI, 0·7% OIT and 1·9% BIT – concentrations corresponding to their individual EC3 values. No statistically significant difference in proliferation of CD4⁺ and CD8⁺ T cells was observed between mice challenged with MI compared with mice challenged with BIT and OIT.

Conclusions

The data indicate cross-reactivity between MI, OIT and BIT, when the potency of the chemical was taken into account in choice of challenge concentration. This means that MI-sensitized individuals may react to OIT and BIT if exposed to sufficient concentrations.

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Volume176, Issue1

January 2017

Pages 176-183

Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

Summary

Background

Objectives

Methods

Results

Conclusions

References

Citing Literature

References

Information

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Cross-reactivity between methylisothiazolinone, octylisothiazolinone and benzisothiazolinone using a modified local lymph node assay

Summary

Background

Objectives

Methods

Results

Conclusions

References

Citing Literature

References

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