Effect of iron on expression of efflux pump (adeABC) and quorum sensing (luxI, luxR) genes in clinical isolates of Acinetobacter baumannii
Farzan Modarresi
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
These authors contributed equally to this work.Search for more papers by this authorOmid Azizi
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
These authors contributed equally to this work.Search for more papers by this authorCorresponding Author
Mohammad Reza Shakibaie
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
Research Center for Infectious Diseases and Tropical Medicine, Kerman, Iran
Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, Iran
These authors contributed equally to this work.Mohammad Reza Shakibaie, Department of Microbiology and Virology, Kerman University of Medical Sciences, End of 22-Bahman BLVD, 76167-14111 Kerman, Iran. e-mail: [email protected]Search for more papers by this authorMohammad Motamedifar
Shiraz HIV/AIDS Research Center, Shiraz, Iran
Departments of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Search for more papers by this authorBehnaz Valibeigi
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Search for more papers by this authorShahla Mansouri
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
Search for more papers by this authorFarzan Modarresi
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
These authors contributed equally to this work.Search for more papers by this authorOmid Azizi
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
These authors contributed equally to this work.Search for more papers by this authorCorresponding Author
Mohammad Reza Shakibaie
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
Research Center for Infectious Diseases and Tropical Medicine, Kerman, Iran
Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, Iran
These authors contributed equally to this work.Mohammad Reza Shakibaie, Department of Microbiology and Virology, Kerman University of Medical Sciences, End of 22-Bahman BLVD, 76167-14111 Kerman, Iran. e-mail: [email protected]Search for more papers by this authorMohammad Motamedifar
Shiraz HIV/AIDS Research Center, Shiraz, Iran
Departments of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Search for more papers by this authorBehnaz Valibeigi
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Search for more papers by this authorShahla Mansouri
Department of Microbiology and Virology, Afzalipour School of Medicine, Kerman, Iran
Search for more papers by this authorAbstract
Resistance-nodulation-division efflux system (RND) adeABC contributes to intrinsic resistance to various drug classes in Acinetobacter baumannii. Similarly, quorum sensing (QS) plays an important role in the biofilm formation and pathogenicity of this bacterium. The aims of this study were to evaluate the influence of iron limitation on the expression of efflux pump (adeABC) genes and QS (luxI, luxR) system by relative quantitative real-time polymerase chain reaction (qRT-PCR). In addition, DNA sequence and phylogenetic relatedness of biofilm-associated protein (Bap) gene was also investigated. Sixty-five multidrug-resistant isolates of A. baumannii were recovered from ICU patients of three hospitals in Kerman, Iran. The isolates were highly resistant to at least 11 antibiotics (MIC ≥64 μg/mL); however, 87% and 89% were susceptible to colistin and tigecycline, respectively (MIC 0.05 μg/mL) (p ≤ 0.05). We detected the presence of RND efflux pump, QS, and bap genes with the frequencies of 92% (adeA), 61.5% (adeB), 84.6% (adeC), 80% (luxI), 61% (luxR), and 66% (bap), respectively. qRT-PCR analysis showed that in some isolates, expression of both adeABC and luxI/R was increased more than fourfold in the presence of low iron (20 μm), suggesting the additional regulatory role of iron on both efflux pump and QS system. Alignment and phylogenetic analysis on the strong biofilm forming isolates confirmed that the fragments amplified were indeed part of bap gene and deduced sequence was similar to A. baumannii K9B410.
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