Effect of Rourea coccinea on ethanol-induced male infertility in Wistar albino rats
Eric Agbodjento
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorCorresponding Author
Jean Robert Klotoé
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Normal High School of Natitingou, National University of Sciences, Technology, Engineering and Mathematics, Natitingou, Benin
Correspondence
Jean Robert Klotoé, PO Box 72, Natitingou, Benin.
Email: [email protected]
Search for more papers by this authorTamègnon Victorien Dougnon
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorTéniola Isabelle Sacramento
School of Management and Operation of Livestock Systems, National University of Agriculture, Ketou, Benin
Search for more papers by this authorTossou Jacques Dougnon
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorJean-Marc Atègbo
Laboratory of Molecular Physiopathology and Toxicology, Department of Animal Physiology, Faculty of Sciences and Techniques (FAST), University of Abomey-Calavi, Abomey-Calavi, Benin
Search for more papers by this authorEric Agbodjento
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorCorresponding Author
Jean Robert Klotoé
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Normal High School of Natitingou, National University of Sciences, Technology, Engineering and Mathematics, Natitingou, Benin
Correspondence
Jean Robert Klotoé, PO Box 72, Natitingou, Benin.
Email: [email protected]
Search for more papers by this authorTamègnon Victorien Dougnon
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorTéniola Isabelle Sacramento
School of Management and Operation of Livestock Systems, National University of Agriculture, Ketou, Benin
Search for more papers by this authorTossou Jacques Dougnon
Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Search for more papers by this authorJean-Marc Atègbo
Laboratory of Molecular Physiopathology and Toxicology, Department of Animal Physiology, Faculty of Sciences and Techniques (FAST), University of Abomey-Calavi, Abomey-Calavi, Benin
Search for more papers by this authorFunding information
This study was funded by the Government of the Republic of Benin through the PhD Student support programme 2017–2018 of the Ministry of Higher Education and Scientific Research.
Abstract
Ethanol consumption is a risk factor of male infertility. The use of medicinal plants offers an alternative for the treatment of male infertility in developing countries. This study aimed to evaluate the Rourea coccinea effect on ethanol-induced male infertility in Wistar rats. Twenty-five (25) male Wistar rats were randomised into five groups of five rats and treated by oesophageal gavage over a 28-day period. Group 1 (negative control) received distilled water; Group 2 (positive control) received 30% ethanol at 7 mg/kg body weight; Group 3 (reference control) received 30% ethanol co-treated with the reference drug, clomiphene citrate; Groups 4 and 5 (test groups) received 30% ethanol co-treated with Rourea coccinea hydro-ethanolic extract at 200 and 400 mg/kg respectively. Testosterone hormone, sperm parameters and testicular histopathology were evaluated. Ethanol treatment induced a significant reduction (p < .05) in sperm count, motility, viability and a significant increase in sperm abnormalities because of the significant decrease (p < .05) in testosterone levels. These data correlate with the alterations observed in the seminiferous tubule on histopathological examination of the testes. However, co-treatment of ethanol with Rourea coccinea extract or the reference drug restored the ethanol-induced toxic effects on the reproductive organs, sperm profile and testosterone level.
CONFLICT OF INTERESTS
The authors declare that they have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that used in this current study are available from the corresponding author upon reasonable request.
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