In vitro bactericidal efficacy of a new triple antibiotic paste formulation against Enterococcus faecalis biofilm
Benjaporn Smittiset DDS
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorDanuchit Banomyong DDS, PhD
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorNisarat Ruangsawasdi DDS, MSc, PhD
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Jittranan Kaewprag DDS, PhD
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Correspondence
Jittranan Kaewprag, Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Yothi Road, Bangkok 10400, Thailand.
Email: [email protected]
Search for more papers by this authorBenjaporn Smittiset DDS
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorDanuchit Banomyong DDS, PhD
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorNisarat Ruangsawasdi DDS, MSc, PhD
Department of Pharmacology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Jittranan Kaewprag DDS, PhD
Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Bangkok, Thailand
Correspondence
Jittranan Kaewprag, Department of Operative Dentistry and Endodontics, Faculty of Dentistry, Mahidol University, Yothi Road, Bangkok 10400, Thailand.
Email: [email protected]
Search for more papers by this authorAbstract
The aim of this study was to evaluate the bactericidal efficacy on Enterococcus faecalis biofilm of a novel, low-concentration triple-antibiotic paste (TAP-L2) in propylene glycol/carboxymethylcellulose vehicle (VEH-2), a ready-to-use prototype, compared with two currently used products; a low-concentration (TAP-L1) and a high-concentration paste (TAP-H1) mixed in macrogol/propylene glycol vehicle (VEH-1). Sixty-two root canals were infected with E. faecalis biofilm, medicated with (a) TAP-L1, (b) TAP-L2, (c) TAP-H1, (d) VEH-1 or (e) VEH-2 (n = 10) and incubated for 21 days. Live/dead assays were performed using confocal laser scanning microscopy (CLSM), and dead bacteria (%) were calculated. The TAP-H1 group demonstrated the highest dead bacteria (67.54 ± 2.38%), which was significantly higher than the TAP-L2 and TAP-L1 groups (56.85 ± 7.11% and 54.23 ± 10.19%) (p < 0.05). The TAP groups demonstrated significantly higher dead bacteria than the VEH-1 and VEH-2 groups (47.51 ± 6.41% and 45.14 ± 8.28%) (p < 0.05). The ready-to-use TAP-L2 had antibacterial activity comparable to TAP-L1, both of which were lower than TAP-H1.
CONFLICT OF INTEREST
The authors declare that they have no conflicts of interest.
REFERENCES
- 1Hoshino E. Information on LSTR 3MIX-MP therapy: Lesion sterilization and tissue repair (LSTR). How to prepare 3MIX-MP. 2007 [cited 2019 Aug 22]. Available from: http://www.lstr.jp/e/information.html
- 2Sato T, Hoshino E, Uematsu H, Noda T. In vitro antimicrobial susceptibility to combinations of drugs on bacteria from carious and endodontic lesions of human deciduous teeth. J Oral Microbiol. 1993; 8(3): 172–6.
- 3Hoshino E, Kurihara-Ando N, Sato I, Uematsu H, Sato M, Kota K, et al. In-vitro antibacterial susceptibility of bacteria taken from infected root dentine to a mixture of ciprofloxacin, metronidazole and minocycline. Int Endod J. 1996; 29(2): 125–30.
- 4Banchs F, Trope M. Revascularization of immature permanent teeth with apical periodontitis: new treatment protocol? J Endod. 2004; 30(4): 196–200.
- 5Jeeruphan T, Jantarat J, Yanpiset K, Suwannapan L, Khewsawai P, Hargreaves KM. Mahidol study 1: comparison of radiographic and survival outcomes of immature teeth treated with either regenerative endodontic or apexification methods: a retrospective study. J Endod. 2012; 38(10): 1330–6.
- 6 American Association of Endodontics. Clinical considerations for regenerative procedure revised 4/1/2018. IOP Publishing American Association of Endodontist (AAE). 2018 [cited 2019 Aug 22]. Available from: https://www.aae.org/specialty/wp-content/uploads/sites/2/2018/06/ConsiderationsForRegEndo_AsOfApril2018.pdf
- 7Devaraj S, Jagannathan N, Neelakantan P. Antibiofilm efficacy of photoactivated curcumin, triple and double antibiotic paste, 2% chlorhexidine and calcium hydroxide against Enterococcus fecalis in vitro. Sci Rep. 2016; 6:24797.
- 8Sundqvist G, Figdor D, Persson S, Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998; 85(1): 86–93.
- 9Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod. 2006; 32(2): 93–8.
- 10Orstavik D, Haapasalo M. Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules. Endod Dent Traumatol. 1990; 6(4): 142–9.
- 11Costerton JW, Stewart PS, Greenberg EP. Bacterial biofilms: a common cause of persistent infections. Science. 1999; 284(5418): 1318–22.
- 12Alyas SM, Fischer BI, Ehrlich Y, Spolnik K, Gregory RL, Yassen GH. Direct and indirect antibacterial effects of various concentrations of triple antibiotic pastes loaded in a methylcellulose system. J Oral Sci. 2016; 58(4): 575–82.
- 13Dunavant TR, Regan JD, Glickman GN, Solomon ES, Honeyman AL. Comparative evaluation of endodontic irrigants against Enterococcus faecalis biofilms. J Endod. 2006; 32(6): 527–31.
- 14Diogenes A, Ruparel N, Teixeira F, Hargreaves K. Translational science in disinfection for regenerative endodontics. J Endod. 2014; 40: S52–S7.
- 15Basu P, Narendrakumar U, Arunachalam R, Devi S, Manjubala I. Characterization and evaluation of carboxymethyl cellulose-based films for healing of full-thickness wounds in normal and diabetic rats. ACS Omega. 2018; 3(10): 12622–32.
- 16Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res. 1987; 66(8): 1375–9.
- 17Sabrah AH, Yassen GH, Gregory RL. Effectiveness of antibiotic medicaments against biofilm formation of Enterococcus faecalis and Porphyromonas gingivalis. J Endod. 2013; 39(11): 1385–9.
- 18Frough Reyhani M, Rahimi S, Fathi Z, Shakouie S, Salem Milani A, Soroush Barhaghi MH, et al. Evaluation of antimicrobial effects of different concentrations of triple antibiotic paste on mature biofilm of Enterococcus faecalis. J Dent Res Dent Clin Dent Prospects. 2015; 9(3): 138–43.
- 19Quintas V, Prada-López I, Tomás CI. Analyzing the oral biofilm using fluorescence-based microscopy: what's in a dye? In: A Méndez-Vilas, editor. Microscopy: advances in scientific research and education. Barcelona: Formatex Research Center; 2014. p. 226–38.
- 20Ricucci D, Siqueira JF Jr. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod. 2010; 36(8): 1277–88.
- 21Portenier I, Waltimo T, Ørstavik D, Haapasalo M. The susceptibility of starved, stationary phase, and growing cells of Enterococcus faecalis to endodontic medicaments. J Endod. 2005; 31(5): 380–6.
- 22Du T, Wang Z, Shen Y, Ma J, Cao Y, Haapasalo M. Effect of long-term exposure to endodontic disinfecting solutions on young and old Enterococcus faecalis biofilms in dentin canals. J Endod. 2014; 40(4): 509–14.
- 23Shuping GB, Orstavik D, Sigurdsson A, Trope M. Reduction of intracanal bacteria using nickel-titanium rotary instrumentation and various medications. J Endod. 2000; 26(12): 751–5.
- 24Shen Y, Stojicic S, Haapasalo M. Antimicrobial efficacy of chlorhexidine against bacteria in biofilms at different stages of development. J Endod. 2011; 37(5): 657–61.
- 25Carreira CM, Dos Santos SSF, Jorge AOC, Lage-Marques JL. Antimicrobial effect of intracanal substances. J Appl Oral Sci. 2007; 15(5): 453–8.
- 26Nalawade TM, Bhat K, Sogi SHP. Bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms. J Int Soc Prev Community Dent. 2015; 5(2): 114–9.
- 27Cruz EV, Kota K, Huque J, Iwaku M, Hoshino E. Penetration of propylene glycol into dentine. Int Endod J. 2002; 35(4): 330–6.
- 28Walter C, Jawor P, Bernimoulin JP, Hägewald S. Moderate effect of enamel matrix derivative (Emdogain® Gel) on Porphyromonas gingivalis growth in vitro. Arch Oral Biol. 2006; 51(3): 171–6.
- 29Li CL, Martini LG, Ford JL, Roberts M. The use of hypromellose in oral drug delivery. J Pharm Pharmacol. 2005; 57(5): 533–46.
