Volume 47, Issue 3 pp. 538-543
Original Research

Effect of different final irrigation protocols on pulp tissue dissolution from an isthmus model

Alfredo Iandolo DDS

Alfredo Iandolo DDS

Department of Medicine and Surgery, University of Salerno, Salerno, Italy

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Massimo Amato DDS, PhD

Massimo Amato DDS, PhD

Department of Medicine and Surgery, University of Salerno, Salerno, Italy

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Dina Abdellatif BDS, MSc

Dina Abdellatif BDS, MSc

Department of Endodontics, Faculty of Dentistry, University of Alexandria, Alexandria, Egypt

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Ana Flávia A. Barbosa DDS, MSc

Ana Flávia A. Barbosa DDS, MSc

Department of Endodontics, School of Dentistry, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil

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Giuseppe Pantaleo DDS, PhD

Giuseppe Pantaleo DDS, PhD

Department of Medicine and Surgery, University of Salerno, Salerno, Italy

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Andrea Blasi DDS, PhD

Andrea Blasi DDS, PhD

Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Naples, Italy

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Vittorio Franco DDS

Vittorio Franco DDS

Dentistry, Faculty of Medicine and Surgery, University of Siena, Siena, Italy

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Emmanuel J.N.L. Silva DDS, MSc, PhD

Corresponding Author

Emmanuel J.N.L. Silva DDS, MSc, PhD

Department of Endodontics, School of Dentistry, Rio de Janeiro State University (UERJ), Rio de Janeiro, RJ, Brazil

Department of Endodontics, School of Dentistry, Grande Rio University (UNIGRANRIO), Rio de Janeiro, RJ, Brazil

Correspondence

Dr. Emmanuel João Nogueira Leal Silva, Rua Herotides de Oliveira, 61/902, Icaraí, Niterói, RJ, Brazil. Email: [email protected]

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First published: 27 April 2021
Citations: 7

Abstract

This study assessed the pulp tissue dissolution from isthmus of a two-rooted maxillary premolar using different final irrigation protocols. After root canal preparation, the surface of the tooth was reduced to an extent that the isthmus could be observed, and 1 mg of pulp tissue was introduced into the isthmus which was covered with a glass slide. Following six groups were tested: syringe and needle; subsonic activation; sonic activation; ultrasonic activation; heating followed by sonic activation; and heating followed by ultrasonic activation. Before and after each experiment a photograph of the isthmus was taken at 30× to register the area of the pulp tissue. Statistical analysis was performed with Kruskal–Wallis and Mann–Whitney tests (P < 0.05). Syringe and needle group showed the lower value of pulp tissue dissolution followed by subsonic irrigation procedures. Pulp tissue dissolution was significantly higher when heating was followed by sonic or ultrasonic activation.

Conflict of interest

The authors certify that they have no commercial or associative interest that represents a conflict of interest in connection with the manuscript.

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