International Journal of Rheumatic Diseases

ORIGINAL ARTICLE

Association of F11R polymorphisms and gene expression with primary Sjögren’s syndrome patients

Tzu-Jung Fang

orcid.org/0000-0002-7318-5152

Division of Geriatrics and Gerontology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

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Ruei-Nian Li,

Ruei-Nian Li

Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan

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Yuan-Zhao Lin,

Yuan-Zhao Lin

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Chia-Hui Lin,

Chia-Hui Lin

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Chia-Chun Tseng,

Chia-Chun Tseng

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Wan-Yu Sung,

Wan-Yu Sung

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Tsan-Teng Ou,

Tsan-Teng Ou

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Cheng-Chin Wu,

Cheng-Chin Wu

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Jeng-Hsien Yen,

Corresponding Author

Jeng-Hsien Yen

[email protected]

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan

Department of Biological Science and Technology, National Chiao-Tung University, Hsinchu, Taiwan

Correspondence

Jeng-Hsien Yen, Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Email: [email protected]

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Tzu-Jung Fang,

Tzu-Jung Fang

orcid.org/0000-0002-7318-5152

Division of Geriatrics and Gerontology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

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Ruei-Nian Li,

Ruei-Nian Li

Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan

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Yuan-Zhao Lin,

Yuan-Zhao Lin

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Chia-Hui Lin,

Chia-Hui Lin

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Chia-Chun Tseng,

Chia-Chun Tseng

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Wan-Yu Sung,

Wan-Yu Sung

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Tsan-Teng Ou,

Tsan-Teng Ou

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Cheng-Chin Wu,

Cheng-Chin Wu

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

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Jeng-Hsien Yen,

Corresponding Author

Jeng-Hsien Yen

[email protected]

Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, Taiwan

Department of Biological Science and Technology, National Chiao-Tung University, Hsinchu, Taiwan

Correspondence

Jeng-Hsien Yen, Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Email: [email protected]

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First published: 29 March 2021

https://doi.org/10.1111/1756-185X.14095

Citations: 1

Tzu-Jung Fang and Ruei-Nian Li equally contributed.

Abstract

Aims

F11R gene encodes junctional adhesion molecule-A protein (JAM-A), which is expressed in various types of cells and is involved in leukocyte extravasation during inflammation. Sjögren's syndrome (SS) is a chronic systemic inflammatory disease that involves lymphocytes invasion of exocrine glands. F11R has been studied in autoimmune diseases, but any association between F11R and SS has not yet been investigated. Therefore, experiments were undertaken to examine the relationships among F11R gene polymorphism, messenger RNA (mRNA) expression and SS patients.

Methods

Three hundred and twenty-nine patients with SS, and 223 healthy controls were enrolled in their recruitment from the Kaohsiung Medical University Hospital. Genomic DNA was extracted from peripheral blood mononuclear cells and gene polymorphisms were genotyped by TaqMan real-time polymerase chain reaction (PCR). F11R mRNA expression was quantitated by quantitative real-time PCR with TaqMan Gene Expression Assay.

Results

Our study showed the genotype -688A/C (rs6695707) was not found in relation to SS patients. The odds ratio of -436A/G (rs12567886) genotype was notably associated with less susceptibility of SS in human leukocyte antigen (HLA)-DR2 negative and HLA-DR3 negative individuals. F11R mRNA expression was lower in SS patients than in the cells of healthy controls.

Conclusion

The result indicated that G allele of -436A/G genotype has the potential protective effect against SS disease condition. F11R mRNA was expressed significantly lower in SS patients.

CONFLICTS OF INTEREST

No potential conflicts of interest were disclosed.

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Association of F11R polymorphisms and gene expression with primary Sjögren’s syndrome patients

Abstract

Aims

Methods

Results

Conclusion

CONFLICTS OF INTEREST

REFERENCES

Citing Literature