Methylation and gene expression of histone deacetylases 6 in systemic lupus erythematosus
Tzu-Jung Fang
Division of Geriatrics and Gerontology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorYuan-Zhao Lin
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorChing-Ching Liu
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorChia-Hui Lin
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorRuei-Nian Li
Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan
Search for more papers by this authorCheng-Chin Wu
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorTsan-Teng Ou
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorWen-Chan Tsai
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorCorresponding Author
Jeng-Hsien Yen
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan
Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, Taiwan
Correspondence: Dr Jeng-Hsien Yen, Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, No. 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan. Email: [email protected]Search for more papers by this authorTzu-Jung Fang
Division of Geriatrics and Gerontology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorYuan-Zhao Lin
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorChing-Ching Liu
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorChia-Hui Lin
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorRuei-Nian Li
Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan
Search for more papers by this authorCheng-Chin Wu
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorTsan-Teng Ou
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorWen-Chan Tsai
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Search for more papers by this authorCorresponding Author
Jeng-Hsien Yen
Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan
Institute of Biomedical Science, National Sun Yat-sen University, Kaohsiung, Taiwan
Correspondence: Dr Jeng-Hsien Yen, Division of Rheumatology, Department of Internal Medicine, Kaohsiung Medical University Hospital, No. 100 Tz-You 1st Road, Kaohsiung City 807, Taiwan. Email: [email protected]Search for more papers by this authorAbstract
Aim
The purpose of this study is to investigate the role of methylation in the histone deacetylases 6 (HDAC6) promoter and HDAC6 messenger RNA (mRNA) expression in the pathogenesis of systemic lupus erythematosus (SLE).
Method
Direct bisulfite-polymerase chain reaction (PCR) sequencing was performed to detect the HDAC6 promoter methylation in 33 patients with SLE and 35 healthy controls. The HDAC6 mRNA expression was measured in 93 SLE patients and 84 healthy controls by using the method of quantitative real-time PCR.
Results
This study demonstrated that the methylation rates at HDAC6-680, -660 and -658 were significantly increased in the SLE patients compared with healthy controls (P = 0.041, 0.034 and 0.029, respectively). The SLE patients also had lower HDAC6 mRNA expression than the controls (P = 0.031). However, there was no significant difference in HDAC6 mRNA expression between patients with active and inactive SLE.
Conclusion
The SLE patients had higher methylation in the HDAC6 promoter and lower HDAC6 mRNA expression than the controls. These changes may be related to the susceptibility of SLE. However, they are not associated with the disease activity of SLE.
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