Antioxidant activities of enzymatic-hydrolysed proteins of dromedary (Camelus dromedarius) colostrum
Corresponding Author
Olfa Oussaief
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Author for correspondence: E-mail: [email protected]
Search for more papers by this authorZeineb Jrad
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorIsabelle Adt
ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), University of Claude Bernard Lyon 1, Equipe Mixte d’Accueil n°3733, IUT Lyon 1, 01000 Bourg en Bresse, France
Search for more papers by this authorMohamed Dbara
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorTouhami Khorchani
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorHalima El-Hatmi
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Department of Food, High Institute of Applied Biology of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorCorresponding Author
Olfa Oussaief
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Author for correspondence: E-mail: [email protected]
Search for more papers by this authorZeineb Jrad
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorIsabelle Adt
ISARA Lyon, BioDyMIA (Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires), University of Claude Bernard Lyon 1, Equipe Mixte d’Accueil n°3733, IUT Lyon 1, 01000 Bourg en Bresse, France
Search for more papers by this authorMohamed Dbara
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorTouhami Khorchani
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorHalima El-Hatmi
Livestock and Wildlife Laboratory, Arid Lands Institute of Medenine, University of Gabes, Medenine, 4119 Tunisia
Department of Food, High Institute of Applied Biology of Medenine, University of Gabes, Medenine, 4119 Tunisia
Search for more papers by this authorAbstract
This work investigated the antioxidant activities of dromedary colostrum proteins before and after hydrolysis by pepsin, trypsin, α-chymotrypsin, pancreatin and papain. The enzymatic hydrolysis affected the degrees of hydrolysis, electrophoretic profiles, molecular weight distribution and hydrophobic/hydrophilic properties of the generated peptides. The antioxidant activities were evaluated using four antioxidant assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging activities, ferric reducing power and ferrous ion chelating activity. Interestingly, the antioxidant activities of dromedary colostrum proteins were enhanced after enzymatic hydrolysis. The highest antioxidant potential was obtained by pancreatic hydrolysates (P ≤ 0.05). These results suggest that dromedary colostrum protein hydrolysates are an important source of natural antioxidant peptides.
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