Volume 48, Issue 12 pp. 1936-1939

CONCISE COMMUNICATION

Simultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers

Purna Dwivedi,

Purna Dwivedi

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

Maharaja Sayajirao University of Baroda, Vadodara, India

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Mukul Sharma,

Mukul Sharma

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

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Purushottam Patel,

Purushottam Patel

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

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Pushpendra Singh,

Corresponding Author

Pushpendra Singh

[email protected]

orcid.org/0000-0001-9453-8669

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

Correspondence

Pushpendra Singh, Indian Council of Medical Health, National Institute of Research in Tribal Health, Nagpur Road P.O. - Garha, Jabalpur – 482 003, Madhya Pradesh, India.

Email: [email protected]

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Purna Dwivedi,

Purna Dwivedi

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

Maharaja Sayajirao University of Baroda, Vadodara, India

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Mukul Sharma,

Mukul Sharma

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

Search for more papers by this author

Purushottam Patel,

Purushottam Patel

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

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Pushpendra Singh,

Corresponding Author

Pushpendra Singh

[email protected]

orcid.org/0000-0001-9453-8669

Indian Council of Medical Research, National Institute of Research in Tribal Health, Jabalpur, India

Correspondence

Pushpendra Singh, Indian Council of Medical Health, National Institute of Research in Tribal Health, Nagpur Road P.O. - Garha, Jabalpur – 482 003, Madhya Pradesh, India.

Email: [email protected]

Search for more papers by this author

First published: 21 October 2021

https://doi.org/10.1111/1346-8138.16165

Citations: 5

Purna Dwivedi and Mukul Sharma contributed equally to this study.

[Correction added on 27 October 2021 after first online publication: The affiliation 1 was corrected]

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Abstract

We have developed a polymerase chain reaction-based method to detect and distinguish Mycobacterium leprae and Mycobacterium lepromatosis using a single set of primers based on a 45-bp difference in the amplicon size of their rpoT gene. This method can also help in detecting the cases of co-infection in a single experiment.

CONFLICT OF INTEREST

None declared.

Supporting Information

REFERENCES

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Volume48, Issue12

December 2021

Pages 1936-1939

Simultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers

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CONFLICT OF INTEREST

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Simultaneous detection and differentiation between Mycobacterium leprae and Mycobacterium lepromatosis using novel polymerase chain reaction primers

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CONFLICT OF INTEREST

Supporting Information

REFERENCES

Citing Literature

References

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