Crystallization and preliminary X-ray diffraction study of lactonohydrolase from Fusarium oxysporum

The lactonohydrolase from the fungus Fusarium oxysporum AKU 3702 was crystallized by the vapour-diffusion procedure in the presence of polyethylene glycol 4000 as a precipitant. The crystals belong to the monoclinic space group P2₁ with unit-cell parameters a = 156, b = 100, c = 94.1 Å, β = 91.7°. Assuming that there are two lactonohydrolase-dimer molecules in the asymmetric unit, the crystal volume per unit molecular mass, V_m, is calculated to be 2.94 ų Da⁻¹.