Characterization and crystallization of DivK, an essential response regulator for cell division and differentiation in Caulobacter crescentus
Abstract
DivK is an essential response regulator involved in the complex signal transduction network required for cell division and cell differentiation in Caulobacter crescentus. Small-angle X-ray scattering analysis was valuable for obtaining single crystals of the DivK recombinant protein. These crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 37.2, b = 40.5, c = 67.1 Å and diffract beyond 1.6 Å on a synchrotron beamline.