Volume 58, Issue 7 pp. 1204-1206

Crystallographic study of a naturally occurring trans-splicing intein from Synechocystis sp. PCC 6803

First published: 15 June 2004
Citations: 1
Zihe Rao, e-mail: [email protected]

Abstract

A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been purified and crystallized using PEG 8K as precipitant. The crystal belongs to space group P3121 or P3221, with unit-cell parameters a = b = 58.5, c = 70.2 Å. It has one molecule per asymmetric unit and diffracts to beyond 2.9 Å under cryoconditions (110 K) using a Cu rotating-anode X-ray generator in-house.

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