Crystallization and preliminary crystallographic analysis of Rieske iron–sulfur protein II (soxF) from Sulfolobus acidocaldarius

An archaeal Rieske iron–sulfur protein has been crystallized for the first time. The genetically constructed soluble form of the soxF protein was expressed in E. coli. It contains a correctly inserted [2Fe–­2S] cluster. The authentic soxF protein is part of a terminal oxidase complex in the respiratory chain of the hyperthermoacidophilic crenarchaeon Sulfolobus acidocaldarius (DSM 639). The enzyme crystallizes in the space group P6₁ or P6₅, with unit-cell parameters a = b = 80.19, c = 75.69 Å. A complete data set has been collected to 1.64 Å resolution at 100 K.