Journal of Pediatric Gastroenterology and Nutrition

Volume 45, Issue 4 pp. 493-496

Short Communications

Immunogenicity of Helicobacter pylori Urease B Protein and DNA Vaccines in a Mouse Model

Rodolfo E Bégué,

Corresponding Author

Rodolfo E Bégué

[email protected]

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

Research Institute for Children, New Orleans, LA

Address correspondence and reprint requests to Rodolfo E. Bégué, MD, Children's Hospital, Infectious Diseases, 200 Henry Clay Ave, New Orleans, LA 70118; (e-mail: [email protected]).Search for more papers by this author

Alma R Cruz,

Alma R Cruz

Research Institute for Children, New Orleans, LA

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Andres Ramgoolam,

Andres Ramgoolam

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

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Mary B Breslin,

Mary B Breslin

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

Research Institute for Children, New Orleans, LA

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Rodolfo E Bégué,

Corresponding Author

Rodolfo E Bégué

[email protected]

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

Research Institute for Children, New Orleans, LA

Alma R Cruz,

Alma R Cruz

Research Institute for Children, New Orleans, LA

Search for more papers by this author

Andres Ramgoolam,

Andres Ramgoolam

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

Search for more papers by this author

Mary B Breslin,

Mary B Breslin

Department of Pediatrics, Louisiana State University Health Sciences Center, New Orleans

Research Institute for Children, New Orleans, LA

Search for more papers by this author

First published: 01 October 2007

https://doi.org/10.1097/MPG.0b013e31806c7bf8

Citations: 4

This work was supported in part by an intramural grant from the Department of Pediatrics, Louisiana State University Health Sciences Center, and Children's Hospital, New Orleans, LA. Presented in part at the Pediatric Academic Societies' 2006 Annual Meeting, San Francisco, CA (abstract no. 751095) and Pediatric Academic Societies' 2007 Annual Meeting, Toronto, ON, Canada (abstract no. 752013).

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ABSTRACT

A vaccine could alleviate major morbidity and mortality associated with Helicobacter pylori infection. We immunized BALB/c mice with 3 doses of a protein or DNA vaccine based on H pylori urease B. Protein alone was immunogenic even after the first dose, whereas DNA did not elicit antibodies after 3 doses. DNA preceding protein (D-P-P) appeared to blunt the response to protein, whereas DNA following protein (P-D-D) shifted from a predominantly T helper 2 (Th2) profile to a balanced Th1:Th2 profile. These preliminary findings may have important implications for the development of an H pylori vaccine.

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Volume45, Issue4

October 2007

Pages 493-496

Immunogenicity of Helicobacter pylori Urease B Protein and DNA Vaccines in a Mouse Model

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Immunogenicity of Helicobacter pylori Urease B Protein and DNA Vaccines in a Mouse Model

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