Article

Growth Suppression of Human Laryngeal Squamous Cell Carcinoma by Adenovirus-Mediated Tissue Factor Pathway Inhibitor Gene 2^†

Yanan Sun MD

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

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Minqiang Xie MD, FACS,

Corresponding Author

Minqiang Xie MD, FACS

[email protected]

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

Dr. Minqiang Xie, Department of Otorhinolaryngology–Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou 510630, P.R. ChinaSearch for more papers by this author

Ming Liu MD,

Ming Liu MD

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

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Dejun Jin MD,

Dejun Jin MD

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

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Peng Li MD,

Peng Li MD

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

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Yanan Sun MD,

Yanan Sun MD

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

Search for more papers by this author

Minqiang Xie MD, FACS,

Corresponding Author

Minqiang Xie MD, FACS

[email protected]

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

Dr. Minqiang Xie, Department of Otorhinolaryngology–Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou 510630, P.R. ChinaSearch for more papers by this author

Ming Liu MD,

Ming Liu MD

Department of Otorhinolaryngology–Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China

Search for more papers by this author

Dejun Jin MD,

Dejun Jin MD

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

Search for more papers by this author

Peng Li MD,

Peng Li MD

Department of Otorhinolaryngology—Head and Neck Surgery, The Third Affiliated Hospital, Sun Yat Sen University, Guangzhou, P.R. China

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First published: 02 January 2009

https://doi.org/10.1097/01.mlg.0000205589.84020.d2

Citations: 13

^†

Supported by a grant from The Science and Technology Foundation of Guangdong Province, China, grant #2003C30309, and a grant from The Natural Science Foundation of China, #30540010.

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Abstract

Objective: The purpose of this study was to explore the inhibiting role of adenoviral-mediated (Ad)-tissue factor pathway inhibitor (TFPI)-2 gene in the growth of laryngeal squamous cell carcinoma (LSCC).

Study Design: The nude mice bearing LSCC were prepared by intracutaneous injection of Hep-2 cell. Gene therapy was performed by injecting adenoviruses carrying TFPI-2 gene around tumors in the animal model.

Methods: Eighteen nude mice bearing Hep-2 cell tumor were randomly separated into the treated group and control group. The former were injected with recombinant adenovirus Ad-TFPI-2 around the tumor, and the later were injected with equivalent Ad-LacZ. After treatment, differences of tumor weight, volume, and ultrastructure of tumor cells between these two groups were observed by measuring and using transmission electron microscope. Apoptosis in Hep-2 xenotransplants was detected using the terminal deoxy-transferase-mediated dUTP nick end labeling. In addition, the expressions of TFPI-2 protein and proliferating cell nuclear antigen (PCNA) in tumor tissues were detected using Western blot and immunohistochemistry, respectively.

Results: The average weight and volume of tumor in the treated group were significantly lower than that in the control group (P < .01). The PCNA index was obviously lower in the tumors treated group than that in the control group (P < .01). In addition, cell apoptosis was observed in xenotransplants of the treated group but not in the control group.

Conclusion: Peritumor injection of Ad-TFPI-2 can inhibit growth of LSCC in nude mice model, and TFPI-2 might be a desirable gene for gene therapy in LSCC.

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Volume116, Issue4

April 2006

Pages 596-601

Growth Suppression of Human Laryngeal Squamous Cell Carcinoma by Adenovirus-Mediated Tissue Factor Pathway Inhibitor Gene 2^†

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Growth Suppression of Human Laryngeal Squamous Cell Carcinoma by Adenovirus-Mediated Tissue Factor Pathway Inhibitor Gene 2†

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Growth Suppression of Human Laryngeal Squamous Cell Carcinoma by Adenovirus-Mediated Tissue Factor Pathway Inhibitor Gene 2^†