Low-dose oral ferrous fumarate aggravated intestinal inflammation in rats with dss-induced colitis
Corresponding Author
Kari Erichsen MD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Department of Medicine, Section for Gastroenterology, Haukeland University Hospital, N-5021 Bergen, NorwaySearch for more papers by this authorAnne Marita Milde PhD
Department of Biological and Medical Psychology, University of Bergen, Bergen, Norway
Search for more papers by this authorGülen Arslan MD, PhD
Institute of Medicine, University of Bergen, Bergen, Norway
National Institute of Nutritional and Seafood Research, Bergen, Norway
Search for more papers by this authorLars Helgeland MD, PhD
Department of Pathology, Haukeland University Hospital, Bergen, Norway
Search for more papers by this authorOddrun Anita Gudbrandsen MS
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorRune J Ulvik MD, PhD
Laboratory of Clinical Biochemistry, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorRolf K Berge PhD
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorTrygve Hausken MD, PhD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorArnold Berstad MD, PhD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorCorresponding Author
Kari Erichsen MD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Department of Medicine, Section for Gastroenterology, Haukeland University Hospital, N-5021 Bergen, NorwaySearch for more papers by this authorAnne Marita Milde PhD
Department of Biological and Medical Psychology, University of Bergen, Bergen, Norway
Search for more papers by this authorGülen Arslan MD, PhD
Institute of Medicine, University of Bergen, Bergen, Norway
National Institute of Nutritional and Seafood Research, Bergen, Norway
Search for more papers by this authorLars Helgeland MD, PhD
Department of Pathology, Haukeland University Hospital, Bergen, Norway
Search for more papers by this authorOddrun Anita Gudbrandsen MS
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorRune J Ulvik MD, PhD
Laboratory of Clinical Biochemistry, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorRolf K Berge PhD
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorTrygve Hausken MD, PhD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorArnold Berstad MD, PhD
Department of Medicine, Haukeland University Hospital, Bergen, Norway
Institute of Medicine, University of Bergen, Bergen, Norway
Search for more papers by this authorAbstract
Background: Oral ferrous iron therapy may reinforce intestinal inflammation. One possible mechanism is by catalyzing the production of reactive oxygen species. We studied the effects of low-dose oral ferrous fumarate on intestinal inflammation and plasma redox status in dextran sulfate sodium (DSS)-induced colitis in rats.
Methods: Forty male Wistar rats were divided into 5 groups: no intervention, sham gavage (distilled water), ferrous fumarate, DSS, and ferrous fumarate + DSS. Ferrous fumarate was dissolved in distilled water (0.60 mg Fe2+/kg per day) and administered by gavage on days 1 to 14. All rats were fed a standard diet. Colitis was induced by 5% DSS in drinking water on days 8 to 14. Rats were killed on day 16. Histologic colitis scores, fecal granulocyte marker protein, plasma malondialdehyde, plasma antioxidant vitamins, and plasma aminothiols were measured.
Results: DSS significantly increased histologic colitis scores (P < 0.001) and fecal granulocyte marker protein (P < 0.01). Ferrous fumarate further increased histologic colitis scores (P < 0.01) in DSS-induced colitis. DSS + ferrous fumarate decreased plasma vitamin A compared with controls (P < 0.01). Otherwise, no changes were seen in plasma malondialdehyde, plasma antioxidant vitamins, or plasma aminothiols.
Conclusion: Low-dose oral ferrous iron enhanced intestinal inflammation in DSS-induced colitis in rats.
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