Failure of 5-HT3 receptors in regulation of ethanol-induced ascorbic acid release in rat striatum
Corresponding Author
Chun-Fu Wu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Dr Chun-Fu Wu, Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110015, PR China. Tel: + 86 24 23903 205; fax: + 86 24 23896050; e-mail: [email protected]Search for more papers by this authorJing Liu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorWen Liu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorSilvana Consolo
Laboratory of Cholinergic System, Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy
Search for more papers by this authorMei Huang
Laboratory of Cholinergic System, Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy
Search for more papers by this authorJing-Yu Yang
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorCorresponding Author
Chun-Fu Wu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Dr Chun-Fu Wu, Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110015, PR China. Tel: + 86 24 23903 205; fax: + 86 24 23896050; e-mail: [email protected]Search for more papers by this authorJing Liu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorWen Liu
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorSilvana Consolo
Laboratory of Cholinergic System, Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy
Search for more papers by this authorMei Huang
Laboratory of Cholinergic System, Istituto di Ricerche Farmacologiche “Mario Negri”, Milan, Italy
Search for more papers by this authorJing-Yu Yang
Department of Pharmacology of Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, PR China
Search for more papers by this authorAbstract
Previous studies have shown that the serotonergic system was involved in ethanol-induced striatal ascorbic acid release in rats. In the present study the possible role of 5-HT 3 receptors in ethanol-induced striatal ascorbic acid release was investigated in rats using 5-HT 3 antagonists ondansetron, DAU 6215 and 5-HT 3 agonist 2-methyl-serotonin. Extracellular level of ascorbic acid in the striatum was determined by means of in vivo microdialysis coupled to HPLC with electrochemical detection. Ethanol (3 g/kg, i.p.) induced a significant increase in ascorbic acid release. Ondansetron (0.2 and 2.0 mg/kg, i.p.), DAU 6215 (0.06, 0.12 and 0.24 mg/kg, i.p.) and 2-methyl-serotonin (250 μg/rat, i.c.v.), administered 10 minutes before 0.15 M NaCl or ethanol (3 g/kg, i.p.), affect neither the basal nor the ethanol-induced ascorbic acid release in rat striatum. 2-Methylserotonin, at a dose of 500 μg/rat, i.c.v., increased the basal, but did not affect the ethanol-induced ascorbic acid release in rat striatum. However, ritanserin (1 mg/kg, s.c.), a 5-HT 2 receptor antagonist, and BIMU 8 (40 μg/rat, i.c.v.), a 5-HT 4 agonist, significantly antagonized ethanol-induced ascorbic acid release. These results suggest that 5-HT 3 receptors, which form a part of cation channels, may not be involved in ethanolinduced striatal ascorbic acid release.
References
- 1 Kasuki H. Vitamin C and nervous tissue in vivo and in vitro aspects. In: JR Harris, editor. Ascorbic acid: biochemistry and biomedical cell biology, subcellular biochemistry, vol. 25. New York : Plenum Press; 1996, pp. 293–311.
- 2 Karanth S, Yu WH, Walczewska A, Mastronardi C, McCann SM. Ascorbic acid acts as an inhibitory transmitter in the hypothalamus to inhibit stimulated luteinizing hormone-releasing hormone release by scavenging nitric oxide. Proc Natl Acad Sci USA 2000; 97: 1891–6.
- 3 Rebec GV, Centore JM, White LK, Alloway KD. Ascorbic acid and the behavioral response to haloperidol: Implications for the action of antipsychotic drugs. Science 1985; 227: 438–40.
- 4 Wu CF, Zhang HL, Liu W. Potentiation of ethanolinduced loss of the righting reflex by ascorbic acid in mice: interaction with dopamine antagonists. Pharmacol Biochem Behav 2000; 65: 413–18.
- 5 Wu CF, Liu W, Liu J, Yeh C-H. dl-Fenfluramine inhibits ethanol-induced ascorbic acid release in rat striatum studied by microdialysis. Addict Biol 1998; 3: 295–308.
- 6 Wu CF, Liu J, Consolo S, Liu W. 5-HT 1Areceptors mediate inhibition of ethanol-induced ascorbic acid release in rat striatum studied by microdialysis. Neurosci Lett 1998; 250: 95–8.
- 7 Saxena PR. Serotonin receptors: subtypes, functional responses and therapeutic relevance. Pharmacol Ther 1995; 66: 339–68.
- 8 Lovinger DM. 5-HT3 receptors and the neural actions of alcohols: an increasing exciting topic. Neurochem Int 1999; 35: 125–30.
- 9 Lovinger DM, Zhou Q. Alcohols potentiate ion current mediated by recombinant 5-HT3RA receptors expressed in a mammalian cell line. Neuropharmacology 1994; 33: 1567–72.
- 10 Zhou Q, Verdoorn TA, Lovinger DM. Alcohols potentiate the function of 5-HT3 receptor-channels on NCB-2-neuroblastoma cells by favouring and stabilazing the open channel state. J Physiol (Lond) 1998; 507: 335–52.
- 11 Nakagawa Y, Iwasaki T. Ethanol-induced statedependent learning is mediated by 5-hydroxytrytamine3 receptors but not by N-methyl-D-aspartate receptor complex. Brain Res 1996; 706: 227–32.
- 12 Matsuzawa S, Suzuki T, Misawa M, Nagase H. Roles of 5-HT3 and opioid receptors in the ethanol-induced place preference in rats exposed to conditioned fear stress. Life Sci 1999; 64: PL241–9.
- 13 Dyr W, Kostowski W. Evidence that the amygdala is involved in the inhibitory effects of 5-HT3 receptor antagonists on alcohol drinking in rats. Alcohol 1995; 12: 387–91.
- 14 Knapp DJ, Pohorecky LA. Zacopride, a 5-HT3 receptor antagonist, reduced voluntary ethanol consumption in rats. Pharmacol Biochem Behav 1992; 41: 847–50.
- 15 Compbell AD, Mcbride WJ. Serotonin-3 receptor and ethanol-stimulated dopamine release in the nucleus accumbens. Pharmacol Biochem Behav 1995; 51: 835–42.
- 16 Wozniak KM, Pert A, Linnoila M. Antagonism of 5-HT3 receptors attenuates the effects of ethanol on extracellular dopamine. Eur J Pharmacol 1990; 187: 287–9.
- 17 Engel SR, Allan AM. 5-HT3 receptor over-expression enhances ethanol sensitivity in mice. Psychoparmacology (Berl) 1999; 144: 411–5.
- 18 Ciccocioppo R, Ge J, Barnes NM, Cooper SJ. Central 5-HT3 receptors in P and in AA alcoholpreferring rats: an autoradiographic study. Brain Res Bull 1998; 46: 311–15.
- 19 Beardsley PM, Lopez OJ, Gullikson G, Flynn D. Serotonin 5-HT3 antagonists fail to affect ethanol self-administration of rats. Alcohol 1994; 11: 389–95.
- 20 Iusco G, Boido V, Sparatore F, et al. New benzamide-derived 5-HT3 receptor antagonists which prevent the effects of ethanol on extracellular dopamine, and fail to reduce voluntary alcohol intake in rats. Farmaco 1997; 52: 141–6.
- 21 Wu CF, Bertorelli R, Sacconi M, Pepeu G, Consolo S. Decrease of brain acetylcholine release in aging freely moving rats detected by microdialysis. Neurobiol Aging 1988; 9: 357–61.
- 22 Consolo S, Arnaboldi S, Giorgi S, Russi G, Ladinsky H. 5-HT4 receptor stimulation facilitates acetylcholine release in rat frontal cortex. Neuroreport 1994; 5: 1230–2.
- 23 Consolo S, Bertorelli R, Russi G, Zambelli M, Ladinsky H. Serotonergic facilitation of acetylcholine release in vivo from rat dorsal hippocampus via serotonin 5-HT3 receptors. J Neurochem 1994; 62: 2254–61.
- 24
Turconi M,
Schiantarelli P,
Borsini F,
Rizzi CA; Ladinsky H,
Donetti A.
Azabicycloalkyl benzimidazolones: Interaction with serotonergic 5-HT3 and 5-HT4 receptors and potential therapeutic implications.
Drugs Fut
1991; 16: 1011–26.
10.1358/dof.1991.016.11.167591 Google Scholar
- 25 Richardson BP, Engel G. The pharmacology and function of 5-HT3 receptors. Trends Neurosci 1986; 9: 424–8.
- 26 Grant KA, Barrett JE. Blockade of the discriminative stimulus effects of ethanol with 5-HT3 receptor antagonists. Psychopharmacology (Berl) 1991; 104: 451–6.
- 27 Bienkowski P, Kuca P, Piasecki J, Kostowski W. 5-HT3 receptor antagonist, tropisetron, does not influence ethanol-induced conditioned taste aversion and conditioned place aversion. Alcohol 1997; 14: 63–9.
- 28 McKinzie DL, Eha R, Cox R, et al. Serotonin3 receptor antagonism of alcohol intake: effects of drinking conditions. Alcohol 1998; 15: 291–8.
- 29 Hope AG, Downie DL, Sutherland L, Lambert JJ, Peters JA, Burcell B. Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit. Eur J Pharmacol 1993; 245: 187–92.
- 30 Singh SP, Handa RK, Depala V, Gao Y, Mellroy PJ, Ravindra R. The effect of ethanol on muscarinic receptor-G protein coupling in the rat cortex. Pharmacol Toxicol 1997; 81: 294–9.
- 31 Singh SP, Gao Y, Kunapuli SP, Ravindra R. Ethanol inhibits G protein-mediated glucose uptake by C6 glioma cells. Neuroreport 1999; 10: 595–9.
- 32 Lu M-R, Wagner GC, Fisher H. Ethanol consumption following acute fenfluramine, fluoxetine, and dietary tryptophan. Pharmacol Biochem Behav 1993; 44: 931–7.
- 33 Zabik JE, Roache JD. 5-hydroxytryptophan-induced conditioned taste aversion to ethanol in the rat. Pharmacol Biochem Behav 1983; 18: 785–90.
- 34 Wilson AW, Neill JC, Costall B. The 5-HT1A receptor agonist 8-OH-DPAT reduces ethanol intake and maintained behaviour in female SD rats. Alcohol 1996; 13: 407–13.
- 35 Richards JB, Sabol KE, Seiden LS. Fluoxetine prevents the disruptive effects of fenfluramine on differential reinforcement of low rate 7 2-second schedule performance. J Pharmacol Exp Ther 1993; 267: 1256–63.
- 36 Gehlert DR, Gackenheimer SL, Wong DT, Robertson DW. Localization of 5-HT3 receptors in the rat brain using [3H]-LY278584. Brain Res 1991; 553: 149–54.
- 37 Verge D, Daval G, Marcinkiewicz M, et al. Quantitative autoradiography of multiple 5-HT1 receptor subtypes in the brain of control or 5,7-dihydroxytryptamine-treated rats. J Neurosci 1986; 6: 3474–82.
- 38 Liu J, Wu CF, Liu W, Zhang HL, Li CL. Involvement of the corticostriatal glutamatergic pathway in ethanol-induced ascorbic acid release in rat striatum. Addict Biol 1999; 4: 273–81.
- 39 Liu W, Wu CF, Huang M, Xiao K. Opposite effects of sulpiride and SCH 23390 on ethanol-induced striatal ascorbic acid release in intact and 6-hydroxydopamine lesioned rats. Brain Res 2000; 869: 31–8.
- 40 Liu W, Wu CF, Liu J, Huang M, Xiao K. Differential effects of acute administration of haloperidol and clozapine on ethanol-induced ascorbic acid release in rat striatum. Eur J Pharmacol 2000; 398: 333–9.
- 41
Rebec GV.
Ascorbate: an antioxidant neuroprotectant and extracellular neuromodulator. In: JR Connor, editor.
Metals and oxidative damage in neurological disorders.
New York
: Plenum Press; 1997, pp.
149–73.
10.1007/978-1-4899-0197-2_9 Google Scholar
- 42
Liang X,
Arvanov VL,
Wang RY.
Inhibition of NMDA-receptor mediated responses in the rat medial prefrontal cortical pyramidal cells by the 5-HT3 receptor agonist SR 57227A and 5-HT: intracellular studies.
Synapse
1998; 29: 257–68.
10.1002/(SICI)1098-2396(199807)29:3<257::AID-SYN8>3.0.CO;2-5 CAS PubMed Web of Science® Google Scholar
