Volume 3, Issue 5 pp. 489-492
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Microinjection of Guanine NucleotideAnalogues into Lily Pollen Tubes Results in Isodiametric TipExpansion

M. Eliáŝ

M. Eliáŝ

Faculty of Sciences, Charles University, Prague, Czech Republic

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F. Cvrĉková

F. Cvrĉková

Faculty of Sciences, Charles University, Prague, Czech Republic

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G. Obermeyer

G. Obermeyer

Inst. of Plant Physiology, Paris-Lodron University, Salzburg, Austria

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V. Žársky

Corresponding Author

V. Žársky

Faculty of Sciences, Charles University, Prague, Czech Republic

Institute ofExperimental Botany, Prague, CzechRepublic

Institute of Experimental Botany Rozvojová 135 CZ 160 00 Praha 6 Czech Republic [email protected]Search for more papers by this author
First published: 14 February 2008
Citations: 9

Abstract

Abstract: Heterotrimeric and small G-proteins aresupposed to participate in tip growth of plant cells. Quantitative changes intip growth rate after introduction of non-hydrolysable guanine nucleotideanalogues (NA) into lily pollen tubes have been interpreted as support for thehypothesis that heterotrimeric G-proteins regulate tube elongation (Ma et al.,1999 IDREF=“R243-11”>11). Here, we report that microinjection of guanineNA into lily pollen tubes causes loss of growth polarity, resulting inisodiametric tip swelling. Our results are compatible with current modelssuggesting an involvement of plant Rho-related small G-proteins (Rop) in themaintenance of pollen tube polarity and in tip morphogenesis.

Abbreviations:

  • ADP-β-S
  • adenosine5′-O-(2-thiodiphosphate)
  • ATP-γ-S
  • adenosine5′-O-(3-thiotriphosphate)
  • GDP-β-S
  • guanosine5′-O-(2-thiodiphosphate)
  • GTP-γ-S
  • guanosine5′-O-(3-thiotriphosphate)
  • NA
  • nucleotideanalogues
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