Volume 5, Issue 4 pp. 571-575
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Microsatellites from the beluga whale Delphinapterus leucas

F. C. Buchanan

F. C. Buchanan

Freshwater Institute, Department of Fisheries and Oceans, 501 University Crescent, Winnipeg R3T 2N6, Manitoba, Canada

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M. K. Friesen

M. K. Friesen

Freshwater Institute, Department of Fisheries and Oceans, 501 University Crescent, Winnipeg R3T 2N6, Manitoba, Canada

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R. P. Littlejohn

R. P. Littlejohn

AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New Zealand

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J. W. Clayton

J. W. Clayton

Freshwater Institute, Department of Fisheries and Oceans, 501 University Crescent, Winnipeg R3T 2N6, Manitoba, Canada

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First published: August 1996
Citations: 35
F. C. Buchanan Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5B5. [email protected]

Abstract

Fifteen microsatellites were isolated from a beluga whale Delphinapterus leucas, genomic library. The microsatellites were amplified in 100 beluga obtained from two widely separated locations. An average of 8.6 alleles per locus were detected and the average heterozygosity was 0.65 with a range of 0.27–0.86. All microsatellites were polymorphic and 13 of the genotype distributions observed were in Hardy-Weinberg equilibrium. It was possible with these microsatellites to assign correctly individual whales to their stock-of-origin 98% of the time. Microsatellites were amplified in 15 other cetaceans with these beluga-derived primers.

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