Involvement of cytotoxic granules in the apoptosis of aplastic anaemia
Dr Tetsuro Nagasaka, Division of Pathology, Clinical Laboratory, Nagoya University Hospital, Tsurumai-cho 65, Showa-ku, Nagoya 466–8560, Japan. E-mail: [email protected]
Abstract
Summary. To study the involvement of cytotoxic granules in apoptosis of aplastic anaemia (AA), we analysed bone marrow clot sections of AA for apoptosis and perforin, granzyme B and T-cell intracellular antigen-1 (TIA-1) expression. Perforin, TIA-1 expression and apoptosis were significantly increased in AA patients compared with control subjects, but granzyme B was not. TIA-1 was significantly correlated with apoptotic cells of AA. No correlation was found between perforin and apoptotic cells of AA. These results suggest that perforin and TIA-1 play a role in the pathogenesis of AA. TIA-1 is implicated as an effector in the apoptosis of AA.
