Volume 114, Issue 1 pp. 84-92

Antisense oligodeoxynucleotides to latent membrane protein 1 induce growth inhibition, apoptosis and Bcl-2 suppression in Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells, but not in EBV-positive natural killer cell lymphoma cells

Toshio Noguchi

Toshio Noguchi

Second Department of Internal Medicine and

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Kazuma Ikeda

Kazuma Ikeda

Second Department of Internal Medicine and

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Kazuhiko Yamamoto

Kazuhiko Yamamoto

Second Department of Internal Medicine and

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Isao Yoshida

Isao Yoshida

Second Department of Internal Medicine and

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Atsuko Ashiba

Atsuko Ashiba

Second Department of Internal Medicine and

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Junjiro Tsuchiyama

Junjiro Tsuchiyama

Second Department of Internal Medicine and

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Katsuji Shinagawa

Katsuji Shinagawa

Second Department of Internal Medicine and

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Tadashi Yoshino

Tadashi Yoshino

Second Department of Pathology, Okayama University Medical School, Okayama, and

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Minoru Takata

Minoru Takata

Department of Immunology, Kawasaki University Medical School, Kurashiki, Japan

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Mine Harada

Mine Harada

Second Department of Internal Medicine and

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First published: 12 January 2002
Citations: 17
Kazuma Ikeda, MD, The Second Department of Internal Medicine, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700–8558, Japan. E-mail: kazikeda@ med.okayama-u.ac.jp

Abstract

Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP-1) is essential for immortalization of B cells by EBV, protects the infected cells from apoptotic cell death and induces Bcl-2 expression. Suppression of LMP-1 expression by antisense oligodeoxynucleotides (AS-oligo) to LMP-1 inhibits proliferation, promotes apoptosis and suppresses Bcl-2 expression in EBV-transformed B cells. However, the function of LMP-1 expression in EBV-positive natural killer (NK) cell lymphoma cells has not been reported previously. We examined the function of LMP-1 in two EBV-positive NK cell lymphoma cell lines (NK-YS and YT) through suppressing LMP-1 expression by AS-oligo to LMP-1. The AS-oligo to LMP-1 suppressed LMP-1 mRNA and protein expression in two EBV-positive NK cell lymphoma cell lines, as well as in an EBV-transformed B-cell line (CMG-1). Proliferation was inhibited, apoptosis was induced and Bcl-2 expression was suppressed in CMG-1 cells, but none of these events were observed in NK-YS or YT cells. These results suggest that proliferation, inhibition of apoptosis and Bcl-2 expression in EBV-positive NK cell lymphoma cells are not directly regulated by LMP-1 as in EBV-transformed B-cell lines, but are probably mediated through other signal transducing systems.

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