Anti-inflammatory effects of apocynin, an inhibitor of NADPH oxidase, in airway inflammation
So Yong Kim
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorKeun-Ai Moon
Division of Allergy, University of Ulsan College of Medicine, Seoul, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorHee-Yeon Jo
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorSookyung Jeong
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorSeung-Han Seon
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorEunyu Jung
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorYou Sook Cho
Division of Allergy, University of Ulsan College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorCorresponding Author
Eunyoung Chun
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Current address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
Dr K-Y Lee, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. E-mail: [email protected]
Dr E Chun, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300, Cheoncheon-dong, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of KoreaM. E-mail: [email protected]
Search for more papers by this authorCorresponding Author
Ki-Young Lee
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Dr K-Y Lee, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. E-mail: [email protected]
Dr E Chun, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300, Cheoncheon-dong, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of KoreaM. E-mail: [email protected]
Search for more papers by this authorSo Yong Kim
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorKeun-Ai Moon
Division of Allergy, University of Ulsan College of Medicine, Seoul, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorHee-Yeon Jo
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
These authors contributed equally to this work.
Search for more papers by this authorSookyung Jeong
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorSeung-Han Seon
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorEunyu Jung
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Search for more papers by this authorYou Sook Cho
Division of Allergy, University of Ulsan College of Medicine, Seoul, Republic of Korea
Search for more papers by this authorCorresponding Author
Eunyoung Chun
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Current address: Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA.
Dr K-Y Lee, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. E-mail: [email protected]
Dr E Chun, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300, Cheoncheon-dong, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of KoreaM. E-mail: [email protected]
Search for more papers by this authorCorresponding Author
Ki-Young Lee
Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, Republic of Korea
Dr K-Y Lee, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 440-746, Republic of Korea. E-mail: [email protected]
Dr E Chun, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300, Cheoncheon-dong, Jangan-Gu, Suwon, Gyeonggi-Do 440-746, Republic of KoreaM. E-mail: [email protected]
Search for more papers by this authorAbstract
Oxidative stress is implicated in the pathogenesis of allergic asthma and remains an attractive target for the prevention of the disease. Herein, we investigated the anti-inflammatory effects of apocynin, a NADPH oxidase (NOX) inhibitor, in both in vitro and in vivo allergen-induced experimental asthma mediated by Th2 hyperresponsiveness. Apocynin showed potential antioxidant activities and inhibitory effects on the activation of redox-sensitive transcription factors, such as NF-κB and AP-1, induced by pro-inflammatory stimuli, such as TNF-α, lipopolysaccharide and Poly I:C, and that inhibited the production of pro-inflammatory cytokines, such as TNF-α, IL-1β and IL-6. In in vivo experimental asthma model, moreover, apocynin significantly attenuated ovalbumin-induced airway hyperresponsiveness and inflammation, as shown by the attenuation of total inflammatory cell and soluble product influx into bronchoalveolar lavage fluid, such as macrophages, eosinophils, IL-4, IL-5, IL-12, IL-13 and TNF-α. Apocynin also significantly reduced lung inflammation in the tissues. Altogether, these results suggest that apocynin may be useful in the treatment of inflammatory diseases induced by oxidative stress through NOX activity.
Supporting Information
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