Functional coupling between vanillate-O-demethylase and formaldehyde detoxification pathway
Makoto Hibi,
Tomonori Sonoki,
Hideo Mori,
Makoto Hibi
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
Search for more papers by this authorTomonori Sonoki
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
Search for more papers by this authorCorresponding Author
Hideo Mori
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
*Corresponding author. Tel.: +81 42 726 2555; fax: +81 42 726 8330., E-mail address: [email protected]Search for more papers by this authorMakoto Hibi,
Tomonori Sonoki,
Hideo Mori,
Makoto Hibi
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
Search for more papers by this authorTomonori Sonoki
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
Search for more papers by this authorCorresponding Author
Hideo Mori
Biofrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahimachi, Machidashi, Tokyo 194-8533, Japan
*Corresponding author. Tel.: +81 42 726 2555; fax: +81 42 726 8330., E-mail address: [email protected]Search for more papers by this authorEdited by S. Silver
Abstract
Pseudomonas putida vanillate-O-demethylase consisting of VanA and VanB was expressed in Escherichia coli strain K-12. Recombinant E. coli strain K-12 cells expressing VanAB efficiently converted vanillate into protocatechuate with glucose consumption. Mutant lacking either pgi or zwf showed higher or lower converting activity than the parental strain, respectively. Formaldehyde, which is the by-product of the demethylation, was converted into formate in the cellular reaction. Formate accumulation was blocked by gene disruption of the E. coli frmA that coded glutathione-dependent formaldehyde dehydrogenase.
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