Calcium near the release site is essential for basal ACh release in Xenopus
Ruxin Li
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorQi Lei
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorGe Song
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorXiangping He
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorCorresponding Author
Zuoping Xie
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Corresponding author. Tel.: +86 10 6278 8677; fax: +86 10 6277 2271. [email protected]Search for more papers by this authorRuxin Li
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorQi Lei
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorGe Song
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorXiangping He
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Search for more papers by this authorCorresponding Author
Zuoping Xie
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, PR China
Corresponding author. Tel.: +86 10 6278 8677; fax: +86 10 6277 2271. [email protected]Search for more papers by this authorAbstract
Extracellular calcium is essential for neurotransmitter release, but the detailed mechanism by which Ca2+ regulates basal synaptic release has not yet been fully explored. In this study, calcium imaging and the whole-cell patch-clamp technique were used to investigate the role of Ca2+ in basal acetylcholine (ACh) release in the Xenopus neuromuscular junction and in isolated myocytes exogenously loaded with ACh. Carried out in normal and Ca2+-free extracellular solution, the results indicate that Ca2+ near the release site is essential for basal neurotransmitter release.
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