Journal of Tissue Engineering and Regenerative Medicine

Volume 15, Issue 12 pp. 1082-1091

RESEARCH ARTICLE

LINC00662 facilitates osteosarcoma progression via sponging miR-103a-3p and regulating SIK2 expression

Jianghu Huang,

Jianghu Huang

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Feiyue Lin,

Feiyue Lin

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Chuncai Xu,

Chuncai Xu

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Yang Xu,

Corresponding Author

Yang Xu

[email protected]

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Correspondence

Yang Xu, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, 350001, Fujian, China; Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, 350001, Fujian, China.

Email: [email protected]

Search for more papers by this author

Jianghu Huang,

Jianghu Huang

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Feiyue Lin,

Feiyue Lin

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Chuncai Xu,

Chuncai Xu

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Search for more papers by this author

Yang Xu,

Corresponding Author

Yang Xu

[email protected]

Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China

Department of Orthopedics, Fujian Provincial Hospital, Fujian Medical University, Fuzhou, Fujian, China

Correspondence

Email: [email protected]

Search for more papers by this author

First published: 24 September 2021

https://doi.org/10.1002/term.3242

Citations: 14

Jianghu Huang and Feiyue Lin are co-first authors.

Share a link

Email
Wechat
Bluesky

Abstract

Long non-coding RNA (lncRNA) involvement in regulating assorted cancers has been determined. Long intergenic non-protein coding RNA 662 (LINC00662) has been studied in gastric cancer. However, its function was not elucidated in osteosarcoma (OS). Thus, we aimed to discover LINC00662 function and the corresponding mechanism in OS. In this study, we found that LINC00662 displayed high expression in OS cells. LINC00662 down-regulation negatively affected OS cell malignant behaviors and tumor growth. Subsequently, miR-103a-3p was proven to bind with LINC00662 and overexpression of miR-103a-3p inhibited OS cell proliferation, migration and invasion. Then, SIK2, the downstream of miR-103a-3p, was up-regulated in OS cells and positively regulated by LINC00662. In addition, knockdown of SIK2 exerted inhibitory effects on proliferative, migratory and invaded capacities of OS cells. More interestingly, miR-103a-3p depletion or SIK2 overexpression restored the impacts of down-regulated LINC00662 on OS cells. In conclusion, LINC00662 could facilitate OS progression via miR-103a-3p/SIK2 axis.

CONFLICT OF INTEREST

The authors declare that no conflict of interest exists in this study.

Open Research

DATA AVAILABILITY STATEMENT

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Supporting Information

REFERENCES

Billiau, A., Edy, V. G., Heremans, H., Van Damme, J., Desmyter, J., Georgiades, J. A., & De Somer, P. (1977). Human interferon: Mass production in a newly established cell line, MG-63. Antimicrobial Agents and Chemotherapy, 12(1), 11–15.
10.1128/AAC.12.1.11
CAS PubMed Web of Science® Google Scholar
Chen, F., Chen, L., Qin, Q., & Sun, X. (2019). Salt-inducible kinase 2: An oncogenic signal transmitter and potential target for cancer therapy. Frontiers in Oncology, 9, 18.
10.3389/fonc.2019.00018
PubMed Web of Science® Google Scholar
Evans, C. H. (2019). The vicissitudes of gene therapy. Bone & Joint Research, 8(10), 469–471.
10.1302/2046-3758.810.BJR-2019-0265
PubMed Web of Science® Google Scholar
Fitzgerald, J. (2020). Applications of CRISPR for musculoskeletal research. Bone & Joint Research, 9(7), 351–359.
10.1302/2046-3758.97.BJR-2019-0364.R2
PubMed Web of Science® Google Scholar
Fogh, J., & Trempe, G. (1975). New human tumor cell lines. In J. Fogh (Ed.), Human tumor cells in vitro (pp. 115–159). Springer US.
10.1007/978-1-4757-1647-4_5
Google Scholar
Harris, S. A., Enger, R. J., Riggs, B. L., & Spelsberg, T. C. (1995). Development and characterization of a conditionally immortalized human fetal osteoblastic cell line. Journal of Bone and Mineral Research: The Official Journal of the American Society for Bone and Mineral Research, 10(2), 178–186.
10.1002/jbmr.5650100203
CAS PubMed Web of Science® Google Scholar
Jimmy, R., Stern, C., Lisy, K., & White, S. (2017). Effectiveness of mifamurtide in addition to standard chemotherapy for high-grade osteosarcoma: A systematic review. JBI Database of Systematic Reviews and Implementation Reports, 15(8), 2113–2152.
10.11124/JBISRIR-2016-003105
PubMed Google Scholar
Li, N., Zhang, L. Y., Qiao, Y. H., & Song, R. J. (2019). Long noncoding RNA LINC00662 functions as miRNA sponge to promote the prostate cancer tumorigenesis through targeting miR-34a. European Review for Medical and Pharmacological Sciences, 23(9), 3688–3698.
CAS PubMed Web of Science® Google Scholar
Li, S., Han, Y., Liang, X., & Zhao, M. (2020). LINC01089 inhibits the progression of cervical cancer via inhibiting miR-27a-3p and increasing BTG2. The Journal of Gene Medicine, 23(1), e3280.
PubMed Web of Science® Google Scholar
Liu, Y., Li, M., Yu, H., & Piao, H. (2020). lncRNA CYTOR promotes tamoxifen resistance in breast cancer cells via sponging miR125a5p. International Journal of Molecular Medicine, 45(2), 497–509.
CAS PubMed Web of Science® Google Scholar
Liu, Z., Yao, Y., Huang, S., Li, L., Jiang, B., Guo, H., Lei, W., Xiong, J., & Deng, J. (2018). LINC00662 promotes gastric cancer cell growth by modulating the Hippo-YAP1 pathway. Biochemical and Biophysical Research Communications, 505(3), 843–849.
10.1016/j.bbrc.2018.09.191
CAS PubMed Web of Science® Google Scholar
Miao, S., Wang, J., Xuan, L., & Liu, X. (2020). LncRNA TTN-AS1 acts as sponge for miR-15b-5p to regulate FBXW7 expression in ovarian cancer. Biofactors. 46(4), 600–607.
10.1002/biof.1622
CAS PubMed Web of Science® Google Scholar
Mumith, A., Coathup, M., Edwards, T. C., Gikas, P., Aston, W., & Blunn, G. (2020). Multidrug chemotherapy causes early radiological signs of loosening in distal femoral replacements. Bone & Joint Research, 9(7), 333–340.
10.1302/2046-3758.97.BJR-2019-0122.R1
PubMed Web of Science® Google Scholar
Pontén, J., & Saksela, E. (1967). Two established in vitro cell lines from human mesenchymal tumours. International Journal of Cancer, 2(5), 434–447.
10.1002/ijc.2910020505
CAS PubMed Web of Science® Google Scholar
Shen, B., Zhou, N., Hu, T., Zhao, W., Wu, D., & Wang, S. (2019). LncRNA MEG3 negatively modified osteosarcoma development through regulation of miR-361-5p and FoxM1. Journal of Cellular Physiology, 234(8), 13464–13480.
10.1002/jcp.28026
CAS PubMed Web of Science® Google Scholar
Simpson, S., Dunning, M. D., de Brot, S., Grau-Roma, L., Mongan, N. P., & Rutland, C. S. (2017). Comparative review of human and canine osteosarcoma: Morphology, epidemiology, prognosis, treatment and genetics. Acta Veterinaria Scandinavica, 59(1), 71.
10.1186/s13028-017-0341-9
PubMed Web of Science® Google Scholar
Sun, S., Chai, S., Zhang, F., & Lu, L. (2019). Overexpressed microRNA-103a-3p inhibits acute lower-extremity deep venous thrombosis via inhibition of CXCL12. IUBMB Life, 72(3), 492–504.
10.1002/iub.2168
PubMed Web of Science® Google Scholar
Wang, H., Li, H., Zhang, L., & Yang, D. (2018). Overexpression of MEG3 sensitizes colorectal cancer cells to oxaliplatin through regulation of miR-141/PDCD4 axis. Biomedicine & Pharmacotherapy, 106, 1607–1615.
10.1016/j.biopha.2018.07.131
CAS PubMed Web of Science® Google Scholar
Wang, H., Yu, M., Hu, W., Chen, X., Luo, Y., Lin, X., Zeng, Y., & Yao, X. (2019). Linc00662 promotes tumorigenesis and progression by regulating miR-497-5p/AVL9 axis in colorectal cancer. Frontiers in Genetics, 10, 1385.
10.3389/fgene.2019.01385
CAS PubMed Web of Science® Google Scholar
Wang, J., Su, Z., Lu, S., Fu, W., Liu, Z., Jiang, X., & Tai, S. (2018). LncRNA HOXA-AS2 and its molecular mechanisms in human cancer. Clinica Chimica Acta; International Journal of Clinical Chemistry, 485, 229–233.
10.1016/j.cca.2018.07.004
CAS PubMed Web of Science® Google Scholar
Wang, K., Ma, L., Tang, J., Yu, Q., Shen, Y., Wei, Y., Zhu, C., Deng, Z., & Zhang, W. (2020). LncRNA00518 promotes cell proliferation through regulating miR-101 in bladder cancer. Journal of Cancer, 11(6), 1468–1477.
10.7150/jca.35710
CAS PubMed Web of Science® Google Scholar
Xu, D., Chen, Y., Yuan, C., Zhang, S., & Peng, W. (2019). Long non-coding RNA LINC00662 promotes proliferation and migration in oral squamous cell carcinoma. OncoTargets and Therapy, 12, 647–656.
10.2147/OTT.S188691
CAS PubMed Web of Science® Google Scholar
Xu, R., Feng, F., Yu, X., Liu, Z., & Lao, L. (2018). LncRNA SNHG4 promotes tumour growth by sponging miR-224-3p and predicts poor survival and recurrence in human osteosarcoma. Cell Proliferation, 51(6), e12515.
10.1111/cpr.12515
PubMed Web of Science® Google Scholar
Yang, X. Z., Cheng, T. T., He, Q. J., Lei, Z. Y., Chi, J., Tang, Z., Liao, Q.-X., Zhang, H., Zeng, L.-S., & Cui, S.-Z. (2018). LINC01133 as ceRNA inhibits gastric cancer progression by sponging miR-106a-3p to regulate APC expression and the Wnt/beta-catenin pathway. Molecular Cancer, 17(1), 126.
10.1186/s12943-018-0874-1
PubMed Web of Science® Google Scholar
Zhang, G., Chen, H. X., Yang, S. N., & Zhao, J. (2020). MAGI1-IT1 stimulates proliferation in non-small cell lung cancer by upregulating AKT1 as a ceRNA. European Review for Medical and Pharmacological Sciences, 24(2), 691–698.
CAS PubMed Web of Science® Google Scholar
Zhang, X., Wang, W., Zhu, W., Dong, J., Cheng, Y., Yin, Z., & Shen, F. (2019). Mechanisms and functions of Long non-coding RNAs at multiple regulatory levels. International Journal of Molecular Sciences, 20(22), 5573.
10.3390/ijms20225573
CAS PubMed Web of Science® Google Scholar

Citing Literature

All articles

Filename	Description
term3242-sup-0001-fig_s1.tif1.5 MB	Figure S1
term3242-sup-0002-table_s1.xlsx11.9 KB	Table S1

LINC00662 facilitates osteosarcoma progression via sponging miR-103a-3p and regulating SIK2 expression

Abstract

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

Information

About Wiley Online Library

Help & Support

Opportunities

Connect with Wiley

LINC00662 facilitates osteosarcoma progression via sponging miR-103a-3p and regulating SIK2 expression

Abstract

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

Related

Information