Preparation and evaluation of gellan gum hydrogel reinforced with silk fibers with enhanced mechanical and biological properties for cartilage tissue engineering
Wooyoup Kim
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJoo Hee Choi
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorPilyun Kim
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJina Youn
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJeong Eun Song
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorAntonella Motta
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy
Search for more papers by this authorClaudio Migliaresi
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy
Search for more papers by this authorCorresponding Author
Gilson Khang
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Jeonju-si, Korea
Correspondence
Gilson Khang, Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center; Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea.
Email: [email protected]
Search for more papers by this authorWooyoup Kim
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJoo Hee Choi
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorPilyun Kim
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJina Youn
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorJeong Eun Song
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Search for more papers by this authorAntonella Motta
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy
Search for more papers by this authorClaudio Migliaresi
Department of Industrial Engineering and BIOtech Research Center, University of Trento, Trento, Italy
Search for more papers by this authorCorresponding Author
Gilson Khang
Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, Jeonju-si, Korea
Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Jeonbuk National University, Jeonju-si, Korea
Correspondence
Gilson Khang, Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center; Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea.
Email: [email protected]
Search for more papers by this authorWooyoup Kim and Joo Hee Choi are first co-authors.
Abstract
Various research about cartilage regeneration using biomaterials has been done recently. Particularly, gellan gum hydrogel (GG) is reported to be suitable as a biomaterial for cartilage tissue engineering (TE) for its water uptaking ability, producibility, and environmental resemblance of native cartilage. Despite these advantages, mechanical and cell adhesion properties are still difficult to modulate. Reinforcement is essential to overcome these problems. Herein, GG was modified by physically blending with different lengths of silk fiber (SF). As SF is expected to improve such disadvantages of GG, mechanical and biological properties were characterized to confirm its reinforcement ability. Mechanical properties such as degradation rate, swelling rate, compression strength, and viscosity were studied and it was confirmed that SF significantly reinforces the mechanical properties of GG. Furthermore, in vitro study was carried out to confirm morphology, biocompatibility, proliferation, and chondrogenesis of chondrocytes encapsulated in the hydrogels. Overall, chondrocytes in the GG blended with SF (SF/GG) showed enhanced cell viability and growth. According to this study, SF/GG can be a promising biomaterial for cartilage TE biomaterial.
CONFLICTS OF INTEREST
The authors declare no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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