Platelet-rich plasma enhances mechanical strength of strattice in rat model of ventral hernia repair
Corresponding Author
Joseph S. Fernandez-Moure
Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA
Correspondence
Joseph S. Fernandez-Moure, Department of Surgery Division of Trauma, Acute and Critical Care Surgery, MS Duke University School of Medicine, Duke University Medical Center DUMC, 2837 2301 Erwin Road, Durham, NC 27710, USA.
Email: [email protected]
Search for more papers by this authorJeffrey L. Van Eps
University of Texas Health Science Center, McGovern Medical School, Houston, Texas, USA
Search for more papers by this authorJacob C. Scherba
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
Search for more papers by this authorIman K. Yazdi
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Medicine, Renal Division, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA
Search for more papers by this authorAndrew Robbins
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorFernando Cabrera
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorCory Vatsaas
Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA
Search for more papers by this authorMichael Moreno
Department of Mechanical Engineering, Texas A&M College of Medicine, Bryan, Texas, USA
Search for more papers by this authorBradley K. Weiner
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorEnnio Tasciotti
IRCCS San Raffaele, University San Raffaele, Rome, Italy
3R Biotech, Milan, Italy
Search for more papers by this authorCorresponding Author
Joseph S. Fernandez-Moure
Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA
Correspondence
Joseph S. Fernandez-Moure, Department of Surgery Division of Trauma, Acute and Critical Care Surgery, MS Duke University School of Medicine, Duke University Medical Center DUMC, 2837 2301 Erwin Road, Durham, NC 27710, USA.
Email: [email protected]
Search for more papers by this authorJeffrey L. Van Eps
University of Texas Health Science Center, McGovern Medical School, Houston, Texas, USA
Search for more papers by this authorJacob C. Scherba
Department of Biomedical Engineering, Duke University, Durham, North Carolina, USA
Search for more papers by this authorIman K. Yazdi
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Medicine, Renal Division, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA
Search for more papers by this authorAndrew Robbins
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorFernando Cabrera
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Search for more papers by this authorCory Vatsaas
Division of Trauma, Acute, and Critical Care Surgery, Department of Surgery, Duke University School of Medicine, Durham, North Carolina, USA
Search for more papers by this authorMichael Moreno
Department of Mechanical Engineering, Texas A&M College of Medicine, Bryan, Texas, USA
Search for more papers by this authorBradley K. Weiner
Department of Nanomedicine, Surgical Advanced Technologies Lab, Houston Methodist Research Institute, Houston, Texas, USA
Department of Orthopedic Surgery, Houston Methodist Hospital, Houston, Texas, USA
Search for more papers by this authorEnnio Tasciotti
IRCCS San Raffaele, University San Raffaele, Rome, Italy
3R Biotech, Milan, Italy
Search for more papers by this author[Corrections added on May 10, 2021 after first online publication: The affiliation and spelling for author Jeffrey L. Van Eps have been corrected. Other affiliations have subsequently been renumbered.]
Abstract
Incisional hernia is a common complication of hernia repair despite the development of various synthetic and bio-synthetic repair materials. Poor long-term mechanical strength, leading to high recurrence rates, has limited the use of acellular dermal matrices (ADMs) in ventral hernia repair (VHR). Biologically derived meshes have been an area of increasing interest. Still these materials bring the risk of more aggressive immune response and fibrosis in addition to the mechanical failures suffered by the synthetic materials. Platelet-rich plasma (PRP), a growth-factor-rich autologous blood product, has been shown to improve early neovascularization, tissue deposition, and to decrease the rates of recurrence. Here, we demonstrate that PRP promotes the release of growth factors stromal derived factor (SDF)-1, transforming growth factor-beta, and platelet-derived growth factor in a dose-dependent manner. Additionally, we utilize an aortic ring angiogenesis assay to show that PRP promotes angiogenesis in vitro. A rat model of VHR using StratticeTM ADM demonstrates similar findings in vivo, corresponding with the increased expression of vascular endothelial growth factor and collagen type 1 alpha 1. Finally, we show that the molecular and cellular activity initiated by PRP results in an increased mechanical stiffness of the hernia repair mesh over time. Collectively, these data represent an essential step in demonstrating the utility and the mechanism of platelet-derived plasma in biomaterial-aided wound healing and provide promising preclinical data that suggest such materials may improve surgical outcomes.
CONFLICT OF INTEREST
The authors have no conflicts of interest to declare.
Open Research
DATA AVAILABILITY STATEMENT
The raw data required to reproduce these findings are available to download https://data.mendeley.com/datasets/znc4tx7rb7/1. The processed data required to reproduce these findings are available to download from https://data.mendeley.com/datasets/znc4tx7rb7/1.
Supporting Information
| Filename | Description |
|---|---|
| term3200-sup-0001-fig_s1.tiff2.9 MB | Supplementary Material 1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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